B7-H2/ICOSLG Fc Chimera, Human
| Synonyms | ICOSLG; B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; ICOS ligand |
| Description | B7-H2, best known as the ligand of inducible co-stimulator, belongs to B7-CD28 family. B7-H2 is a transmembrane glycoprotein of approximately 60 kDa and is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes. It’s a ligand for CD28 and CTLA-4 in human, whereas these interactions are not conserved in mouse. B7-H2 and B7-1 or B7-2 interacts with CD28 through distinctive domains. B7-H2-CD28 interaction is essential for the co-stimulation of human T cells' primary responses to allogeneic antigens and memory recall responses. Recombinant Human B7-H2 Fc Chimera produced in HEK293 cells. It’s a polypeptide chain containing 473 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhB7-H2 has a molecular mass of 70-80 kDa, analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
| Accession No | O75144 |
| Species | Human |
| Source | HEK293 |
| Biological Activity | Immobilized ICOS Fc Chimera, Human(Cat:Z03412) at 5 μg/mL (100 μL/well) can bind Biotin-B7-H2/ICOSLG Fc Chimera, Human with a linear range of 0.76-12.21ng/mL when detected by Streptavidin-HRP second antibody.. |
| Sequence | DTQEKEVR AMVGSDVE LSCACPEG SRFDLNDV YVYWQTSE SKTVVTYH IPQNSSLE NVDSRYRN RALMSPAG MLRGDFSL RLFNVTPQ DEQKFHCL VLSQSLGF QEVLSVEV TLHVAANF SVPVVSAP HSPSQDEL TFTCTSIN GYPRPNVY WINKTDNS LLDQALQN DTVFLNMR GLYDVVSV LRIARTPS VNIGCCIE NVLLQQNL TVGSQTGN DIGERDKI TENPVSTG EKNAATWS |
| Measured Molecular Weight | 70-80 kDa, observed by reducing SDS-PAGE. |
| Purity | > 97% as analyzed by reducing SDS-PAGE. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol. |
| Reconstitution | Reconstituted in ddH2O at 100 μg/mL. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant B7-H2 Fc Chimera, Human remains stable for up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human B7-H2 Fc should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
Immobilized ICOS Fc Chimera, Human(Cat:Z03412) at 5 μg/mL (100 μL/well) can bind Biotin-B7-H2/ICOSLG Fc Chimera, Human with a linear range of 0.76-12.21ng/mL.
Cat. No. |
Product Name |
Price |
|
|---|---|---|---|
Z03409-50 |
B7-1(CD80) Fc Chimera, Human - 50 ug |
$159.00 |
|
Z03416-1 |
B7-2/CD86 Fc Chimera, Human - 1 mg |
$1200.00 |
|
Z03452-1 |
B7-2/CD86 Fc His, Human - 1 mg |
$1200.00 |
|
Z03415-1 |
B7-H2/ICOSLG His, Human - 1 mg |
$1200.00 |
|
Z03426-100 |
B7-H3 Fc Chimera, Human - 100 ug |
$275.00 |
|
Z03427-100 |
B7-H3, His, Human - 100 ug |
$395.00 |
For more documents, please visit "Technical Support".
| Cat. No. Z03414 | ||
|---|---|---|
Size |
Price |
|
| 100 μg | $250.00 | |
| 1 mg | $1200.00 | |
