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B7-H2/ICOSLG Fc Chimera, Human

*This product has been discontinued!*
B7-H2, best known as the ligand of inducible co-stimulator, belongs to B7-CD28 family. B7-H2 is a transmembrane glycoprotein of approximately 60 kDa and is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes. It’s a ligand for CD28 and CTLA-4 in human, whereas these interactions are not conserved in mouse. B7-H2 and B7-1 or B7-2 interacts with CD28 through distinctive domains. B7-H2-CD28 interaction is essential for the co-stimulation of human T cells' primary responses to allogeneic antigens and memory recall responses.Recombinant Human B7-H2 Fc Chimera produced in HEK293 cells. It’s a polypeptide chain containing 473 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhB7-H2 has a molecular mass of 70-80 kDa, analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03414
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Product Introduction
Species Human
Protein Construction
B7-H2/ICOSLG (Asp19-Ser258)
Accession # O75144		 hFc
N-term C-term
Purity > 97% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity Immobilized ICOS, hFc, Human (Cat. No.: Z03412) at 5.0 μg/ml (100 µl/well) can bind biotinylated B7-H2/ICOSLG, hFc, Human when detected by Streptavidin-HRP second antibody.
Expression System HEK 293
Apparent Molecular Weight 70~80 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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Background
Target Background B7-H2, best known as the ligand of inducible co-stimulator, belongs to B7-CD28 family. B7-H2 is a transmembrane glycoprotein of approximately 60 kDa and is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes. It’s a ligand for CD28 and CTLA-4 in human, whereas these interactions are not conserved in mouse. B7-H2 and B7-1 or B7-2 interacts with CD28 through distinctive domains. B7-H2-CD28 interaction is essential for the co-stimulation of human T cells' primary responses to allogeneic antigens and memory recall responses.Recombinant Human B7-H2 Fc Chimera produced in HEK293 cells. It’s a polypeptide chain containing 473 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhB7-H2 has a molecular mass of 70-80 kDa, analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Synonyms ICOSLG; B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; ICOS ligand
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.