B7-2/CD86 Fc Chimera, Human
| Synonyms | Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2 |
| Description | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs), both are type 1 transmembrane proteins with a membrane distal IgV and a membrane proximal IgC domain. They share ~25% sequence homology and interact with the same receptors, CD28 and CTLA-4.Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. T cell activation requires engagement of the T cell receptor (TCR) with the peptide–MHC complex presented on the cell surface of antigen presenting cells (APCs). In addition to this antigen-specific interaction, a second interaction involving costimulatory receptors (CD28, ICOS) on T cells and their respective ligands (B7-1/B7-2, ICOS-L) on APCs is required for optimal T cell activation. B7-1 and B7-2 may also function to deliver signal into dendritic cells. While B7-1 favors binding to CTLA-4, B7-2 shows a preference for CD28. Recombinant Human B7-2 Fc Chimera produced in HEK293 cells is a polypeptide chain containing 461 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhB7-2 has a molecular mass of 65-80 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
| Accession No | P42081 |
| Species | Human |
| Source | HEK293 |
| Biological Activity | Immobilized B7-2CD86/-Fc at 5 μg/mL (100 μL/well) can bind human Biotin-CD28-Fc with a linear range of 0.09~3.12μg/mL when detected by Streptavidin-HRP. |
| Sequence | LSGAAPLK IQAYFNET ADLPCQFA NSQNQSLS ELVVFWQD QENLVLNE VYLGKEKF DSVHSKYM GRTSFDSD SWTLRLHN LQIKDKGL YQCIIHHK KPTGMIRI HQMNSELS VLANFSQP EIVPISNI TENVYINL TCSSIHGY PEPKKMSV LLRTKNST IEYDGVMQ KSQDNVTE LYDVSISL SVSFPDVT SNMTIFCI LETDKTRL LSSPFSIE LEDPQPPP DHIP |
| Measured Molecular Weight | 65-80 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% as analyzed by reducing SDS-PAGE. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant B7-2 Fc Chimera, Human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human B7-2 Fc Chimera should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
Immobilized B7-2/CD86-Fc at 5 μg/mL (100 μL/well) can bind human Biotin-CD28-Fc with a linear range of 0.09~3.12μg/mL.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03409-50 |
B7-1(CD80) Fc Chimera, Human - 50 ug |
$159.00 |
|
Z03452-1 |
B7-2/CD86 Fc His, Human - 1 mg |
$1200.00 |
|
Z03414-1 |
B7-H2/ICOSLG Fc Chimera, Human - 1 mg |
$1200.00 |
|
Z03415-1 |
B7-H2/ICOSLG His, Human - 1 mg |
$1200.00 |
|
Z03426-100 |
B7-H3 Fc Chimera, Human - 100 ug |
$275.00 |
|
Z03427-100 |
B7-H3, His, Human - 100 ug |
$395.00 |
For more documents, please visit "Technical Support".
| Cat. No. Z03416 | ||
|---|---|---|
| Size | Price | |
| 100 μg | $250.00 | |
| 1 mg | $1200.00 | |
