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Home » Gene Synthesis & Molecular Biology Services » SIRPα Fc Chimera, Human

SIRPα Fc Chimera, Human

Synonyms CD172 antigen-like family member A; CD172a antigen; CD172a
Description Signal regulatory protein alpha (SIRPα, designated CD172a), is also known as CD172 antigen-like family member A (CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), which is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPα is Ubiquitous and highly expressed in brain. SIRPA/CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA/SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function by similarity. SIRPα recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production. Recombinant Human SIRPα Fc Chimera produced in HEK293 cells is a polypeptide chain containing 572 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule; rhSIRPα a molecular mass of 85-100 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P78324
Species Human
Source HEK293
Biological Activity Immobilized CD47, His, Human (Cat.No.Z03419) at 2 μg/mL (100 µl/well), can bind SIRPa Fc Chimera, Human with a linear range of 0.25-185 ng/mL.
Sequence
EEELQVIQ PDKSVLVA AGETATLR CTATSLIP VGPIQWFR
GAGPGREL IYNQKEGH FPRVTTVS DLTKRNNM DFSIRIGN
ITPADAGT YYCVKFRK GSPDDVEF KSGAGTEL SVRAKPSA
PVVSGPAA RATPQHTV SFTCESHG FSPRDITL KWFKNGNE
LSDFQTNV DPVGESVS YSIHSTAK VVLTREDV HSQVICEV
AHVTLQGD PLRGTANL SETIRVPP TLEVTQQP VRAENQVN
VTCQVRKF YPQRLQLT WLENGNVS RTETASTV TENKDGTY
NWMSWLLV NVSAHRDD VKLTCQVE HDGQPAVS KSHDLKVS
AHPKEQGS NTAAENTG SNER

Measured Molecular Weight 85-100 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant SIRPα remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution; Human SIRPα should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Note For research use only

  • SIRPα Fc Chimera, Human
  • SIRPα Fc Chimera, Human

    Immobilized CD47, His, Human (Cat.No.Z03419) at 2 μg/mL (100 µl/well), can bind SIRPa Fc Chimera, Human with a linear range of 0.25-185 ng/mL.


Cat. No.
Product Name
Price
Z03421-1
SIRPα, His, Human - 1 mg
$1500.00
 


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Cat. No. Z03420
Size
Price
100 μg $250.00
1 mg $1200.00