SIRPα Fc Chimera, Human
| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as analyzed by SDS-PAGE | ||||
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | ||||
| Biological Activity | Immobilized CD47, His, Human (Cat.No.: Z03419) at 2.0 μg/ml (100 µl/well), can bind SIRPα, hFc, Human with a linear range of 0.25-185.0 ng/ml. | ||||
| Expression System | HEK 293 | ||||
| Apparent Molecular Weight | 85~100 kDa, on SDS-PAGE under reducing conditions. | ||||
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol. | ||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. | ||||
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Immobilized CD47, His, Human (Cat.No.Z03419) at 2 μg/mL (100 µl/well), can bind SIRPa Fc Chimera, Human with a linear range of 0.25-185 ng/mL.
| Target Background | Signal regulatory protein alpha (SIRPα, designated CD172a), is also known as CD172 antigen-like family member A (CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), which is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPα is Ubiquitous and highly expressed in brain. SIRPA/CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA/SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function by similarity. SIRPα recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production. |
| Synonyms | CD172 antigen-like family member A; CD172a antigen; CD172a |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03421-1 |
SIRPα, His, Human - 1 mg |
$1500.00 |
For more documents, please visit "Technical Support".
| Cat. No. Z03420 | ||
|---|---|---|
| Size | Price | |
| 100 μg | $250.00 | |
| 1 mg | $1200.00 | |
