SIRPα Fc Chimera, Human
| Synonyms | CD172 antigen-like family member A; CD172a antigen; CD172a |
| Description | Signal regulatory protein alpha (SIRPα, designated CD172a), is also known as CD172 antigen-like family member A (CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), which is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPα is Ubiquitous and highly expressed in brain. SIRPA/CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA/SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function by similarity. SIRPα recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production. Recombinant Human SIRPα Fc Chimera produced in HEK293 cells is a polypeptide chain containing 572 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule; rhSIRPα a molecular mass of 85-100 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
| Accession No | P78324 |
| Species | Human |
| Source | HEK293 |
| Biological Activity | Immobilized CD47, His, Human (Cat.No.Z03419) at 2 μg/mL (100 µl/well), can bind SIRPa Fc Chimera, Human with a linear range of 0.25-185 ng/mL. |
| Sequence | EEELQVIQ PDKSVLVA AGETATLR CTATSLIP VGPIQWFR GAGPGREL IYNQKEGH FPRVTTVS DLTKRNNM DFSIRIGN ITPADAGT YYCVKFRK GSPDDVEF KSGAGTEL SVRAKPSA PVVSGPAA RATPQHTV SFTCESHG FSPRDITL KWFKNGNE LSDFQTNV DPVGESVS YSIHSTAK VVLTREDV HSQVICEV AHVTLQGD PLRGTANL SETIRVPP TLEVTQQP VRAENQVN VTCQVRKF YPQRLQLT WLENGNVS RTETASTV TENKDGTY NWMSWLLV NVSAHRDD VKLTCQVE HDGQPAVS KSHDLKVS AHPKEQGS NTAAENTG SNER |
| Measured Molecular Weight | 85-100 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% as analyzed by reducing SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant SIRPα remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution; Human SIRPα should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
Immobilized CD47, His, Human (Cat.No.Z03419) at 2 μg/mL (100 µl/well), can bind SIRPa Fc Chimera, Human with a linear range of 0.25-185 ng/mL.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z03421-1 |
SIRPα, His, Human - 1 mg |
$1500.00 |
For more documents, please visit "Technical Support".
| Cat. No. Z03420 | ||
|---|---|---|
| Size | Price | |
| 100 μg | $250.00 | |
| 1 mg | $1200.00 | |
