|Synonyms||Transforming growth factor beta-2, TGFB2, Polyergin, G-TSF,Glioblastoma-derived T-cell suppressor factor,Cetermin,BSC-1 cell growth inhibitor,TGF-beta-2|
|Description||Transforming growth factor beta-2 (TGF-β2) is a secreted protein which belongs to the TGF-beta family. It is known as a cytokine that performs many cellular functions and has a vital role during embryonic development. The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. Defects in TGFB2 may be a cause of non-syndromic aortic disease (NSAD).
Recombinant human TGF-β2 produced by a mammalian expression system is a polypeptide chain containing 112 amino acids. A fully biologically active molecule; rh TGF-β2 a molecular mass of 12.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 <0.2 ng/ml, measured in a cell proliferation assay using mouse HT-2 cells.|
Ala³⁰³-Ser⁴¹⁴ (Accession #:P61812);
|Measured Molecular Weight||12.7 kDa; observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.|
|Reconstitution||Reconstituted in ddH₂O at 100 μg/ml.|
|Endotoxin Level||< 1 EU/μg; determined by LAL method.|
|Storage||Lyophilized recombinant TGF-β2, human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution; Human TGF-β2 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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