|Synonyms||TGF-beta-1; CED; DPD1; TGFB; TGF-b1; TGFB1; CEDLAP;latency-associated peptide; TGFbeta; TGF-beta 1 protein; transforming growth factor beta-1|
|Description||Transforming growth factor beta 1 (TGFβ1) is the prototype of a growing superfamily of peptide growth factors and plays a prominent role in a variety of cellular processes, including cell-cycle progression, cell differentiation, reproductive function, development, motility, adhesion, neuronal growth, bone morphogenesis, wound healing, and immune surveillance. TGF-β1, TGF-β2 and TGF-β3 signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-β receptor type II (TβR-II), and a TGF-β receptor type I (TβR-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-β expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, hematopoietic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.
Recombinant Mouse TGF-β1 produced by a mammalian expression system is a polypeptide chain containing 112 amino acids. A fully biologically active molecule; rm TGF-β1 a molecular mass of 12.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 <0.2 ng/ml, measured in a cell proliferation assay using mouse HT-2 cells.|
Ala²⁷⁹-Ser³⁹⁰ (Accession #:P04202);
|Measured Molecular Weight||12.8 kDa; observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in of 4 mM HCl.|
|Reconstitution||Reconstituted in ddH₂O at 100 μg/ml.|
|Endotoxin Level||< 1 EU/μg; determined by LAL method.|
|Storage||Lyophilized recombinant TGF-β1, Mouse remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution; Mouse TGF-β1 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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