Catalog Products » Recombinant Proteins » PVR/CD155, His, Human

PVR/CD155 His, Human

*This product has been discontinued!*
PVR is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates. PVR's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. PVR/CD155 was originally isolated based on its ability to mediate polio virus attachment to host cells. The full length (or PVR alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20aa signal sequence, a 323aa extracellular region, a 24aa TM segment and a 50aa cytoplasmic tail. PVR binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically. PVR is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin.
Z03436
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Product Introduction
Species Human
Protein Construction
PVR/CD155 (Trp21-Asn343)
Accession # P15151		 Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity Immobilized CD155 His, Human (Cat. No.: Z03436) at 5.0 μg/ml (100 µl/well) can bind TIGIT Fc, Human (Cat. No.: Z03439) with a linear range of 2.5-10.0 μg/ml.
Expression System HEK 293
Apparent Molecular Weight 50~65 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background PVR is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates. PVR's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. PVR/CD155 was originally isolated based on its ability to mediate polio virus attachment to host cells. The full length (or PVR alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20aa signal sequence, a 323aa extracellular region, a 24aa TM segment and a 50aa cytoplasmic tail. PVR binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically. PVR is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin.
Synonyms CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.