Catalog Products » 2B4/CD244, hFc, Human
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2B4/CD244 Fc Chimera, Human

CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. This gene encodes a cell surface receptor expressed on natural killer cells (NK cells) (and some T cells) mediating non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. CD244 can also be expressed on non-lymphocytes such as eosinophils, mast cells and dendritic cells.
$120.00
Z03445-50

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Product Introduction
Species Human
Protein Construction
2B4/CD244 (Cys22-Arg221)
Accession # Q9BZW8		 hFc
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity Immobilized SLAMF2 (Mammalian,C-mFc) at 1.0 µg/ml, 2.0 µg/ml(100 μl/well) can bind 2B4/CD244, hFc, Human with EC50=48.56 ng/ml, EC50=75.13 ng/ml when detected by Mouse Anti Human IgG Fc-HRP.
Expression System HEK 293
Apparent Molecular Weight ~73.6 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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2B4/CD244 Fc Chimera, Human

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2B4/CD244 Fc Chimera, Human

»

2B4/CD244 Fc Chimera, Human

Immobilized SLAMF2 (Mammalian,C-mFc) at 1 µg/ml, 2 µg/ml(100 μl/well) can bind 2B4/CD244 Fc Chimera, Human with EC50=48.56 ng/ml, EC50=75.13 ng/ml when detected by Mouse Anti Human IgG Fc-HRP. Background was subtracted from data points before curve fitting. »

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Background
Target Background CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. This gene encodes a cell surface receptor expressed on natural killer cells (NK cells) (and some T cells) mediating non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. CD244 can also be expressed on non-lymphocytes such as eosinophils, mast cells and dendritic cells.
Synonyms NAIL; NKR2B4; Nmrk; SLAMF4; CD244 molecule; CD_antigen: CD244
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.