|Description||GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Human GITR Ligand consists of a 50 amino acid cytoplasmic domain, a 21 aa transsmembrane segment, and a 128 aa extracellular domain (ECD). Within the ECD, human GITR Ligand shares 56% and 60% aa sequence identity with mouse and rat GITR Ligand, respectively. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. GITRL/TNFSF18 is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells.
Recombinant Human GITR Ligand Fc Chimera produced in HEK293 cells is a polypeptide chain containing 359 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhGITRL has a molecular mass of 50 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Immobilized human GITR Fc, Human (Z03440) at 5 μg/mL (100 μL/well) can bind human Biotin-GITR Ligand with a linear range of 48-390 ng/mL when detected by Streptavidin-HRP.|
Glu⁷⁴-Ser¹⁹⁹(Accession #: Q9UNG2), expressed with a C-terminal human IgG1 Fc fragment
ETAKEPCM AKFGPLPS KWQMASSE PPCVNKVS DWKLEILQ
NGLYLIYG QVAPNANY NDVAPFEV RLYKNKDM IQTLTNKS
KIQNVGGT YELHVGDT IDLIFNSE HQVLKNNT YWGIILLA
|Molecular Weight||50 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.1 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant GITR Ligand Fc Chimera, Human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human GITR Ligand Fc Chimera should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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