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Home » Gene Synthesis & Molecular Biology Services » GITR Ligand Fc Chimera, Human

GITR Ligand Fc Chimera, Human

Synonyms TNFSF18,AITRL,TL6,hGITRL,GITR Ligand
Description GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Human GITR Ligand consists of a 50 amino acid cytoplasmic domain, a 21 aa transsmembrane segment, and a 128 aa extracellular domain (ECD). Within the ECD, human GITR Ligand shares 56% and 60% aa sequence identity with mouse and rat GITR Ligand, respectively. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. GITRL/TNFSF18 is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells.
Recombinant Human GITR Ligand Fc Chimera produced in HEK293 cells is a polypeptide chain containing 359 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhGITRL has a molecular mass of 50 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Source HEK293
Biological Activity Immobilized human GITR Fc, Human (Z03440) at 5 μg/mL (100 μL/well) can bind human Biotin-GITR Ligand with a linear range of 48-390 ng/mL when detected by Streptavidin-HRP.
Sequence
Glu⁷⁴-Ser¹⁹⁹(Accession #: Q9UNG2), expressed with a C-terminal human IgG1 Fc fragment
ETAKEPCM AKFGPLPS KWQMASSE PPCVNKVS DWKLEILQ
NGLYLIYG QVAPNANY NDVAPFEV RLYKNKDM IQTLTNKS
KIQNVGGT YELHVGDT IDLIFNSE HQVLKNNT YWGIILLA
NPQFIS

Molecular Weight 50 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant GITR Ligand Fc Chimera, Human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human GITR Ligand Fc Chimera should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.




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Cat. No. Z03446
Size
Price
100 ug $250.00
1 mg $1500.00