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Home » Gene Synthesis & Molecular Biology Services » B7-2/CD86 Fc His, Human

B7-2/CD86 Fc His, Human

Synonyms Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2;CD86; B7-2; B70; CD28LG2; LAB72; MGC34413
Description B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. B7-2 promotes the stabilization of CD28 in the IS, while The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response. Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses
Recombinant Human B7-2/CD86 produced in HEK293 cells is a polypeptide chain containing 228 amino acids with C-terminal 6His. A fully biologically active molecule, rhB7-2/CD86 has a molecular mass of 45-55 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P42081
Species Human
Source HEK293
Biological Activity Immobilized B7-2 His, Human at 5μg/mL (100 μL/well) can bind CTLA-4 Fc, Human with a linear range of 0.977-7.813ng/mL.
Immobilized B7-2 His, Human at 5μg/mL (100 μL/well) can bind CD28 Fc, Human with a linear range of 0.78-6.25μg/mL.
Sequence
LSGAAPLK IQAYFNET ADLPCQFA NSQNQSLS ELVVFWQD
QENLVLNE VYLGKEKF DSVHSKYM GRTSFDSD SWTLRLHN
LQIKDKGL YQCIIHHK KPTGMIRI HQMNSELS VLANFSQP
EIVPISNI TENVYINL TCSSIHGY PEPKKMSV LLRTKNST
IEYDGVMQ KSQDNVTE LYDVSISL SVSFPDVT SNMTIFCI
LETDKTRL LSSPFSIE LEDPQPPP DHIPHHHH HH

Measured Molecular Weight 45-55 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant B7-2/CD86 remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, Human B7-2/CD86 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles
Note For research use only


Cat. No.
Product Name
Price
Z03409-50
B7-1(CD80) Fc Chimera, Human - 50 ug
$159.00
Z03416-1
B7-2/CD86 Fc Chimera, Human - 1 mg
$1200.00
Z03414-1
B7-H2/ICOSLG Fc Chimera, Human - 1 mg
$1200.00
Z03415-1
B7-H2/ICOSLG His, Human - 1 mg
$1200.00
Z03426-100
B7-H3 Fc Chimera, Human - 100 ug
$275.00
Z03427-100
B7-H3, His, Human - 100 ug
$395.00
 



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Cat. No. Z03452
Size
Price
100 μg $250.00
1 mg $1200.00