SARS-CoV-2 Spike protein (S1)
Western blot analysis of SARS-CoV-2 Spike protein (S1)
(GenScript, Z03501) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03501
Lane 2: 5ng of Z03501
Immobilized SARS-CoV-2 Spike protein (S1) (Cat. No. Z03501) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No. Z03484) with a serial dilution.
Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).
Lane 1: 2μg of SARS-CoV-2 Spike protein (S1), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (S1), non-reducing(NR)
> 85% as analyzed by SDS-PAGE
| Species | SARS-CoV-2 | |
| Protein Construction |
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| Purity | > 90% as analyzed by SDS-PAGE | |
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | |
| Biological Activity | SARS-CoV-2 Spike protein (S1) can bind with Human ACE2 in functional ELISA assay. | |
| Expression System | 293 Cells | |
| Theoretical Molecular Weight | 78.3 kDa | |
| Formulation | Supplied as a solution in PBS pH 7.4 | |
| Concentration | Please refer to the COA for the specific lot. | |
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles. |
Western blot analysis of SARS-CoV-2 Spike protein (S1)
(GenScript, Z03501) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03501
Lane 2: 5ng of Z03501
Immobilized SARS-CoV-2 Spike protein (S1) (Cat. No. Z03501) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No. Z03484) with a serial dilution.
Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).
Lane 1: 2μg of SARS-CoV-2 Spike protein (S1), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (S1), non-reducing(NR)
> 85% as analyzed by SDS-PAGE
| Target Background | SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. S protein plays an important role in the induction of neutralizing-antibodies and T-cell responses, as well as protective immunity. |
| Synonyms | SARS-CoV-2 S1 protein; 2019-nCoV S1 protein |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Yuta Kyosei, et al. Ultrasensitive Detection of SARS-CoV-2 Spike Proteins Using the Thio-NAD Cycling Reaction: A Preliminary Study before Clinical Trials. Microorganisms. (2021-10)
Hongguang Liang, et al. ARRB2 promotes colorectal cancer growth through triggering WTAP. Acta Biochim Biophys Sin (Shanghai). (2021-01)
Hicks SM, et al. A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. The Journal of Infectious Diseases. (2021-01)
Julia A Zakashansky, et al. Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes. medrxiv. (2020-11)
Zeng C, et al. Leveraging mRNA Sequences and Nanoparticles to Deliver SARS-CoV-2 Antigens In Vivo. Advanced materials (Deerfield Beach, Fla.). (2020-10)
Yuta Kyosei, et al. Proposal of De Novo Antigen Test for COVID-19: Ultrasensitive Detection of Spike Proteins of SARS-CoV-2. Diagnostics (Basel). (2020)
Kirill Gorshkov, et al. Development of a high-throughput homogeneous AlphaLISA drug screening assay for the detection of SARS-CoV-2 Nucleocapsid. biorxiv. (2020)
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. Z03501 | ||
|---|---|---|
| Size | Price | |
| 100 μg | $380.00 | |
| 1 mg | $2100.00 | |
