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LIGHT, Human

LIGHT, Human

LIGHT, Human

Immobilized LIGHT, Human (Z03560) at 2 μg/ml (100 μl/well) can bind HVEM-Fc, Human with EC50=0.738 µg/ml when detected by Mouse Anti–Human IgG FC-HRP. Background was subtracted from data points before curve fitting.

LIGHT, Human

LIGHT, also known as tumor-necrosis factor (TNF) superfamily member 14 (TNFSF14), is predominantly expressed on activated immune cells and some tumor cells. LIGHT (homologous to lymphotoxin, exhibits inducible expression and competes with Herpes Simplex Virus glycoprotein D for Herpes Virus Entry Mediator, a receptor expressed by T cells), is a protein primarily expressed on activated T cells, activated Natural Killer (NK) cells, and immature dendritic cells (DC). LIGHT can function as both a soluble and cell surface-bound type II membrane protein and must be in its homotrimeric form to interact with its two primary functional receptors: Herpes Virus Entry Mediator (HVEM) and Lymphotoxin-β Receptor (LTβR). LIGHT signaling through these receptors have distinct functions that are cell-type dependent, but interactions with both types of receptors have immune-related implications in tumor biology.
Z03560
$159.00

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Product Introduction
Species Human
Protein Construction
LIGHT (Asp74 - Val240)
Accession # O43557
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity Immobilized LIGHT, Human at 2.0 μg/ml (100 μl/well) can bind HVEM-Fc, Human with EC50=0.738 µg/ml when detected by Mouse Anti-Human IgG FC-HRP.
Expression System HEK 293
Apparent Molecular Weight ~15.6 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • LIGHT, Human
    • LIGHT, Human

  • LIGHT, Human
    • LIGHT, Human

  • LIGHT, Human
    • LIGHT, Human

    Immobilized LIGHT, Human (Z03560) at 2 μg/ml (100 μl/well) can bind HVEM-Fc, Human with EC50=0.738 µg/ml when detected by Mouse Anti–Human IgG FC-HRP. Background was subtracted from data points before curve fitting.


Background
Target Background LIGHT, also known as tumor-necrosis factor (TNF) superfamily member 14 (TNFSF14), is predominantly expressed on activated immune cells and some tumor cells. LIGHT (homologous to lymphotoxin, exhibits inducible expression and competes with Herpes Simplex Virus glycoprotein D for Herpes Virus Entry Mediator, a receptor expressed by T cells), is a protein primarily expressed on activated T cells, activated Natural Killer (NK) cells, and immature dendritic cells (DC). LIGHT can function as both a soluble and cell surface-bound type II membrane protein and must be in its homotrimeric form to interact with its two primary functional receptors: Herpes Virus Entry Mediator (HVEM) and Lymphotoxin-β Receptor (LTβR). LIGHT signaling through these receptors have distinct functions that are cell-type dependent, but interactions with both types of receptors have immune-related implications in tumor biology.
Synonyms CD258 antigen; CD258; HVEM-L, LTg, TNFSF14, TR2, HVEML

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.