> 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC
|Endotoxin Level||< 1 EU/μg of protein by LAL method|
|Biological Activity||The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of 1.0 × 107 IU/mg.|
|Expression System||E. coli|
|Theoretical Molecular Weight||6.1 kDa|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.|
|Reconstitution||It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.|
|Storage & Stability||Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.|
|Target Background||Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. It is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature rat EGF shares 70 % a.a. sequence identity with mature human EGF.|
|Synonyms||Epidermal growth factor; Urogastrone, URG|
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
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