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PDGF-BB, Human

Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).
$120.00
Z03707-10

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Product Introduction
Species Human
Protein Construction
PDGF-BB (Ser82-Thr190)
Accession # P01127
Purity ≥ 98% as analyzed by SDS-PAGE
Endotoxin Level ≤ 0.2 EU/μg of protein by gel clotting method
Biological Activity Measured in a cell proliferation assay using BALB/c 3T3 cells, the ED50 for this effect is less than 10 ng/ml
Expression System E. coli
Theoretical Molecular Weight 24.8 kDa
Apparent Molecular Weight ~25 kDa, on SDS-PAGE under non-reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in 20 mM NaAc-HAc, pH 4.5.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. Avoid repeated freeze-thaw cycles.
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PDGF-BB, Human

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PDGF-BB, Human

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PDGF-BB, Human

The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is 5.42 ng/ml. »

PDGF-BB, Human

Lane 1: 2 μg of PDGF-BB, Human, non-reducing (N)
Lane 2: 2 μg of PDGF-BB, Human, reducing (R)
≥ 98% as analyzed by SDS-PAGE »

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Background
Target Background Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).
Synonyms PDGFBB; PDGF-BB
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.