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FGF-basic, Salmon

Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomyocytes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.
$74.00
Z03727-10

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Product Introduction
Species Salmon
Protein Construction
FGF-basic (Full length)
Accession # XP_035600424.1
Purity ≥ 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity EC50 < 1.0 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the EC50, the calculated specific activity is approximately > 1.0 × 106 U/mg.. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
Expression System E. coli
Theoretical Molecular Weight 17.1 kDa
Apparent Molecular Weight ~20 kDa, on SDS-PAGE under non-reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in 7.8 mM Na2HPO4, 1.5 mM KH2PO4, 2.7 mM KCl, 500 mM NaCl.
Reconstitution Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml.
Storage & Stability Upon receiving, the lyophilized product remains stable for 6 months at lower than -70℃. Upon reconstitution, the product is stable for 1 week at 4℃ or for 3 months at -20℃. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20℃.
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FGF-basic, Salmon

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FGF-basic, Salmon

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FGF-basic, Salmon

The EC50 as determined by a cell proliferation assay using mice Balb/c 3T3 cells is 0.053 ng/ml. »

FGF-basic, Salmon

Lane 1: 2 µg of FGF-basic, Salmon, reducing (R)

Lane 2: 2 µg of FGF-basic, Salmon, non-reducing (NR)

The purity is greater than 95% as analyzed by SDS-PAGE

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Background
Target Background Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomyocytes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.
Synonyms FGF-2; BFGF; FGFB; FGF basic;
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.