Catalog Products » Insulin, Bovine
Insulin, Bovine

Insulin, Bovine

The ED50 as determined by a cell proliferation assay using human MCF7 cells is 0.14 μg/ml.

Insulin, Bovine

Lane 1: 2 µg of Insulin, Bovine, reducing (R)
Lane 2: 2 µg of Insulin, Bovine, non-reducing (NR)
The purity is greater than 95% as analyzed by SDS-PAGE

Insulin, Bovine

Insulin is a peptide hormone produced exclusively by beta cells of the pancreatic islets. The mature form of insulin is a heterodimer of a B chain and an A chain linked by two disulfide bonds. The amino acid sequence of insulin is strongly conserved and varies only slightly between species. Bovine insulin differs from human in only three amino acid residues, and porcine insulin in one. Insulin is considered to be the main anabolic hormone of the body and regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes. Recombinant insulin has been widely used for treatment of diabetes.
Z03735
$395.00

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Product Introduction
Species Bovine
Protein Construction
Insulin (Phe25-Ala54 & Gly85-Asn105)
Accession # P01317
Purity ≥ 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.1 EU/μg of protein by gel clotting method
Biological Activity ED50 < 1.00 μg/ml as determined by a dose-response proliferation assay using human MCF7 epithelial cells. Based on the ED50, the calculated specific activity is approximately > 1× 103 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
Expression System P. pastoris
Theoretical Molecular Weight 5.72 kDa
Apparent Molecular Weight ~6 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in 10 mM Citric acid, pH 2.0-3.0.
Reconstitution Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 1 mg/ml.
Storage & Stability Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.

Examples
  • Insulin, Bovine
    • Insulin, Bovine

  • Insulin, Bovine
    • Insulin, Bovine

    The ED50 as determined by a cell proliferation assay using human MCF7 cells is 0.14 μg/ml.

  • Insulin, Bovine
    • Insulin, Bovine

    Lane 1: 2 µg of Insulin, Bovine, reducing (R)
    Lane 2: 2 µg of Insulin, Bovine, non-reducing (NR)
    The purity is greater than 95% as analyzed by SDS-PAGE


Background
Target Background Insulin is a peptide hormone produced exclusively by beta cells of the pancreatic islets. The mature form of insulin is a heterodimer of a B chain and an A chain linked by two disulfide bonds. The amino acid sequence of insulin is strongly conserved and varies only slightly between species. Bovine insulin differs from human in only three amino acid residues, and porcine insulin in one. Insulin is considered to be the main anabolic hormone of the body and regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes. Recombinant insulin has been widely used for treatment of diabetes.
Synonyms Insulin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.