EGF, Chicken

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.

Price	$190.00
Cat. No.	Z03787-100
Size	100 μg 1 mg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Chicken

Protein Construction

EGF (Gly976-Ala1030)
Accession # A0A8V0XEN7

Purity

≥ 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.2 ng/ml, measured by a cell proliferation assay using BALB/3T3 cells, corresponding to a specific activity of > 5.0 × 10⁶ U/mg.

Expression System

P. pastoris

Theoretical Molecular Weight

6.2 kDa

Apparent Molecular Weight

~ 8.0 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized from a 0.2 μm filtered solution in 1×PBS, pH 7.2

Reconstitution

Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml

Storage & Stability

Upon receiving, this product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 1 week at 4℃ or for 3 months at -20℃. Avoid repeated freeze-thaw cycles.

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Lane 1: 2 µg of EGF, Chicken under reducing condition (R)

Lane 2: 2 µg of EGF, Chicken under non-reducing condition (NR) (N)

The purity is greater than 95% as analyzed by SDS-PAGE

The ED₅₀ as determined by a cell proliferation assay using BALB/3T3 cells is 0.03128 ng/ml. »

Background

Target Background	EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Synonyms	Urogastrone; URG; epidermal growth factor

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For research use only. Not intended for human and animal therapeutic or diagnostic use.