DLL3, His, Human

DLL3, a transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands, inhibits primary neurogenesis and helps divert neurons along specific differentiation pathways. It also contributes to the formation of somite boundaries during paraxial mesoderm segmentation.

Price	$375.00
Cat. No.	Z03813-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

His tag	DLL3 (Ala27-Arg490) Accession # Q9NYJ7-1
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1 EU per μg by the LAL method.

Biological Activity

Immobilized DLL3, His, Human (Cat.No.: Z03813) at 0.1 μg/ml can bind Anti-DLL3 Antibody.

Expression System

HEK293

Theoretical Molecular Weight

50.4 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 200 mM Arginine, pH 7.4.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the lyophilized product remains stable for 6 months at -20℃ or below as supplied from the date of receipt. -80℃ for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

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DLL3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Anti-DLL3 Antibody captured on CM5 Chip via Protein A can bind DLL3, His, Human with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). »

Immobilized DLL3, His, Human at 0.1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA. »

Background

Target Background	DLL3, a transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands, inhibits primary neurogenesis and helps divert neurons along specific differentiation pathways. It also contributes to the formation of somite boundaries during paraxial mesoderm segmentation.
Synonyms	Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.