Wnt-3a hFc Chimera, Human

Wnt-3a activates the canonical Wnt signaling pathway and is expressed in the dorsal midline region, playing a crucial role in spinal cord development. Additionally, it regulates autophagy, apoptosis, neuron regeneration, neurogenic inflammation, and axon regeneration. Wnt-3a promotes the beta-catenin/Tcf pathway, which can induce tumors and lead to cancer in specific cell populations. Moreover, it is widely used in cytokine for organoid construction.

Price	$375.00
Cat. No.	Z03815-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species	Human
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin Level	Less than 1 EU per μg by the LAL method.
Biological Activity	Wnt-3a hFc Chimera, Human (Cat.No.: Z03815) can induce Topflash reporter activity in HEK293T human embryonic kidney cells.
Expression System	HEK293
Theoretical Molecular Weight	58.50 kDa.
Apparent Molecular Weight	Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability	Upon receiving, the product remains stable for 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

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Wnt-3a hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2-12 ng/mL. »

Background

Target Background

Synonyms

Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.