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Annexin V/ANXA5, Human

Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.
$210.00
Z05025-100

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Product Introduction
Species Human
Protein Construction
Annexin V/ANXA5 (Ala2-Asp320)
Accession # P08758
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Expression System E.coli
Theoretical Molecular Weight 35.9 kDa
Apparent Molecular Weight The protein has a predicted MW of 35.9 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 50mM Tris, 240mM NaCl (pH 8.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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Annexin V/ANXA5, Human

The purity of Annexin V/ANXA5, Human is greater than 95% as determined by SEC-HPLC. »

Annexin V/ANXA5, Human

Annexin V/ANXA5, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.
Synonyms Annexin A5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; PP4; PAP-I; VAC-alpha; ANX5; ENX2; ANXA5; HEL-S-7; RPRGL3
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.