R spondin 1/RSPO1, His, Mouse

The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.

Price	$470.00
Cat. No.	Z06333-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Mouse

Protein Construction

R spondin 1/RSPO1 (Ser21-Gln265)_x000D_ Accession # Q9Z132	His
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

28.3 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5).

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test). »

Immobilized R spondin 1/RSPO1, His, Mouse, His Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11 μg/ml determined by ELISA. »

R spondin 1/RSPO1, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Synonyms	R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.