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BMP-2, Human

Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction.
$749.00
Z06829-100

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Product Introduction
Species Human
Protein Construction
BMP-2 (Gln283-Arg396)
Accession # P12643
Purity ≥95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg by the LAL method
Biological Activity The EC50 ≤ 0.02 μg/mL, Measured by its ability to induce alkaline phosphatase production in ATDC-5 cells
Expression System CHO
Theoretical Molecular Weight ~26 kDa, on SDS-PAGE under non-reducing conditions
Formulation Lyophilized from 0.22 μm filtered solution 4 mM KH2PO4, 16 mM Na2HPO4, 800 mM NaCl, 5% trehalose, 5% Mannitol, pH 7.4.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20°C or below. Upon reconstitution, the product should be stable for 1 week at 2~8°C, and for 3 months at -20°C or below. Avoid repeated freeze-thaw cycles.
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BMP-2, Human

Figure 1. The functional assay of BMP-2, human (Z06829), measured as EC50 in μg/mL, determined by its ability to induce alkaline phosphatase activities in ADTC-5 cells. The red trace is the control sample in its lyophilized form stored at -80 °C whereas the blue trace is the reconstituted sample in ddH₂O and then stored at 4 °C for 7 days. (No significant loss of activities were observed.) »

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Background
Target Background Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction.
Synonyms BMP-2; BMP2A; Bone morphogenetic protein 2; BMP-2A; BMP2
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