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Expression of sheep pathogen Babesia sp. Xinjiang rhoptry-associated protein 1 and evaluation of its diagnostic potential by enzyme-linked immunosorbent assay.

Parasitology. 2016; 
Niu Q, Liu Z, Yang J, Yu P, Pan Y, Zhai B, Luo J, Guan G, Yin H.
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Recombinant Proteins 1017/S0031182016001293 Qingli Niu and others containing recombinant protein were analysed by SDS–10% PAGE and Western blotting using anti- His6 serum (THETM His Tag Antibody, mAb, Mouse, GenScript, A00186). Get A Quote


Ovine babesiosis is one of the most important tick-borne haemoparasitic diseases of small ruminants. The ovine parasite Babesia sp. Xinjiang is widespread in China. In this study, recombinant full-length XJrRAP-1aα2 (rhoptry-associated protein 1aα2) and C-terminal XJrRAP-1aα2 CT of Babesia sp. Xinjiang were expressed and used to evaluate their diagnostic potential for Babesia sp. Xinjiang infections by indirect enzyme-linked immunosorbent assay (ELISA). Purified XJrRAP-1aα2 was tested for reactivity with sera from animals experimentally infected with Babesia sp. Xinjiang and other haemoparasites using Western blotting and ELISA. The results showed no cross-reactivities between XJrRAP-1aα2 CT and sera from ... More


Babesia sp. Xinjiang; expression; rhoptry-associated protein; serodiagnosis