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Catalog Antibody> | … SARS-CoV-2 (2019-nCoV) spike S1 + S2 Protein (ECD, His Tag) (Sino Biologicals, 40589- V08B1), and SARS-CoV-2 spike protein (ECD, His & Flag Tag) (GenScript Z03481) … signaling, 4355S). Secondary antibodies were, Anti-His-HRP (Genscript, A00612), Avidin-HRP … | Get A Quote |
We show that SARS-CoV-2 spike protein interacts with cell surface heparan sulfate and angiotensin converting enzyme 2 (ACE2) through its Receptor Binding Domain. Docking studies suggest a putative heparin/heparan sulfate-binding site adjacent to the domain that binds to ACE2. In vitro, binding of ACE2 and heparin to spike protein ectodomains occurs independently and a ternary complex can be generated using heparin as a template. Contrary to studies with purified components, spike protein binding to heparan sulfate and ACE2 on cells occurs codependently. Unfractionated heparin, non-anticoagulant heparin, treatment with heparin lyases, and purified lung heparan sulfate potently block spike protein binding and inf... More