Research Grade AAV Packaging

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Overview

Adeno-associated virus (AAV) is a nonenveloped, single-stranded DNA virus that can infect both nondividing and dividing cells. AAV is thought to be nonpathogenic to humans and only replicates in the presence of a helper virus. These features have made AAV a useful tool for gene delivery to a wide variety of cell types and an attractive vector for gene therapy.

As a professional AAV services vendor, GenScript can seamless connect upstream AAV plasmid construction and downstream AAV packaging. Besides, with GenScript’s proprietary AAV ITR guarantee technology and proprietary strain, we can ensure your AAV packaging reach to higher titer and transduction performance.

Cell Engineering

Highlight

Multiple Serotype

Up to 18 serotypes available

One Stop Shop

Seamless connect plasmid construction and AAV packaging

High Quality AAV Particles

Ultra-purified AAV particles for animal experiments

Workflow

Service Specifications

Serotypes		Volume	Titer	Purity	Endotoxin	TAT	QC Methods
Serotypes		Volume	Titer	Purity	Endotoxin	TAT	Potency	Purity	Impurity
Normal yield serotypes	AAV1, 3b, 5, 7, 8, 9, rh10, PHP.eB, DJ, retro, PHP.S, Olig001. etc	0.2-1 mL, Customized volume	>1x10^13 GC/ml	>95% by SDS-PAGE	< 10 EU/mL	2-3 wks	qPCR (Free) ddPCR (Additional) TCID₅₀ (Additional) GOI Sequencing (Additional) Capsid Titer-ELISA (Additional)	SDS-PAGE (Free) TEM (Additional) AUC (Additional)	Endotoxin test (Free) Mycoplasma test (Additional) Sterility test (Additional) Residual Plasmid test (Additional)
Low yield serotypes	AAV2, 4, 6, 6.2, 7m8, ShH10, AAVA6 [Y705F, Y731F]	0.2-1 mL, Customized volume	>1x10^13 GC/ml	>95% by SDS-PAGE	< 10 EU/mL	2-3 wks		SDS-PAGE (Free) TEM (Additional) AUC (Additional)

Note:
1. Inserted sequence above the length of 4.7kb (5’ITR to 3’ ITR), titer is not guaranteed
2. Packaging of customer-supplied plasmids, titer is not guaranteed

Default QC

SDS-Page

Endotoxin

qPCR
Additional QC

Mycoplasma Test

TEM

TCID₅₀

Capsid titer

Sanger Sequencing

AUC

Get in Touch with GenScript AAV Packaging Services

Case Study

Case study 1: AAV purity test

SDS-PAGE analysis of different AAV serotypes with silver staining

(Purified virions were achieved by chromatography)
Case study 2: AAV empty/full ratio detection

Empty / Full Ratio

Titer: 1x10^12-13 gc/ml (qPCR)

Resources

Comparison of different viral vectors

Characteristic	AAV	Retrovirus	Lentivirus
Genome	4.8kb(ssDNA)	9.7kb (ssRNA)	9kb (ssRNA)
Packaging Capacity	4.7kb	3kb	9kb
Infection	Most dividing and non-dividing cells	Only infect mitotically active cells	Most dividing and non-dividing cells
Transduction Efficiency	Moderate	High	Moderate
Integration	Non-integrating	Integrating	Integrating
Expression	Transient or stable	Stable	Stable or Transient
Immunogenicity	Very Low	High	Low
Biosafety	BSL-1	BSL-2	BSL-2
Gene Therapy Strategy	In vivo & In vitro	Ex vivo	In vitro & Ex vivo

AAV Serotype Recommendation

Tissue type	Recommended AAV Serotype
Central Nervous System (CNS)	AAV1, AAV5, AAV8, AAV9, AAV-DJ
Heart	AAV1, AAV8, AAV9
Kidney	AAV2, AAV9
Liver	AAV8, AAV9, AAV-DJ
Lung	AAV5, AAV6, AAV9
Pancreas	AAV8
RPE (Retinal Pigment Epithelium)	AAV1, AAV2, AAV5, AAV8
Skeletal Muscle	AAV1, AAV6, AAV8, AAV9

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