Resources » Technical Resource Centers » Gene Technical Resources » Molecular Cloning Center » Restriction Digest Protocol
Component
|
Final Concentration/amount
|
---|---|
water
|
to 50 µL
|
buffer
|
1X
|
BSA
|
0.05 units/µL
|
DNA template
|
1 µg
|
Restriction enzyme
|
5-10 U per µg of DNA template (should not exceed 10% of reaction volume)
|