Mutant Libraries (Protein Engineering)

GenScript combines its expertise in de novo gene synthesis and site-directed mutagenesis into an excellent site-directed mutagenesis library construction service. The site-directed mutagenesis library offers a great platform for protein function and active center studies. In these libraries, any given residue can be substituted with any of other 19 common amino acids, creating systematic combinations of amino acid mutations that reveal any significant pattern.

The final delivery of our site-directed mutagenesis library includes the following:

• Up to 10 µg of mutation constructs
• Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library
• Separated variants
• Sequence verification information
• Statistical analysis of nucleotide distribution at the mutation sites

Scanning point mutation is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning by replacing each amino acid with all 20 amino acids simultaneously. This technique provides a detailed profile of each amino acid at the position. For each codon of interest, a small, site-saturated library is constructed. This library can be delivered as a pool or in a separated format for any substitution variant (19 in total). The application of GenScript's expertise in de novo gene synthesis to the field of sequential permutation scanning allows us to provide superb sequential permutation scanning library construction services.

The final delivery of our scanning point mutation library includes the following:

• Up to 10 µg of mutation constructs
• Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library
• Separated variants (optional)
• Sequence verification information
• Statistical analysis of nucleotide distribution at the mutation sites