pESC-URA

It is a yeast episomal plasmid designed for co-expression of two recombinant proteins in yeast (S. cerevisiae), driven by GAL1 and GAL10 promoters (induced by galactose). This vector contains a URA3 gene for selection in yeast and a Flag tag at MCS1 and a myc tag at MCS2 for recombinant protein detection and purification.

Basic Information

Vector Type: Yeast
Inducer: Galactose
Selection marker (eukaryotic): URA3
Bacterial Resistance(s): Ampicillin
Copy number: High

Component Information

ORI: pUC/pBR322/pMB1, 2μ
Promoter1: GAL10 promoter(-)
Promoter2: GAL1 promoter
Promoter3: T7 promoter
C-tag: FLAG/Myc

Reference

Gou, Y., Li, D., Zhao, M., Li, M., Zhang, J., Zhou, Y., Xiao, F., Liu, G., Ding, H., Sun, C., Ye, C., Dong, C., Gao, J., Gao, D., Bao, Z., Huang, L., Xu, Z., & Lian, J. (2024). Intein-mediated temperature control for complete biosynthesis of sanguinarine and its halogenated derivatives in yeast. Nature Communications, 15(1), 5238. https://doi.org/10.1038/s41467-024-49554-w
Ozber, N., Carr, S. C., Morris, J. S., Liang, S., Watkins, J. L., Caldo, K. M., Hagel, J. M., Ng, K. K. S., & Facchini, P. J. (2022). Alkaloid binding to opium poppy major latex proteins triggers structural modification and functional aggregation. Nature Communications, 13(1), 6768. https://doi.org/10.1038/s41467-022-34313-6
Kedziora, S., Gali, V. K., Wilson, R. H., Clark, K. R., Nieduszynski, C. A., Hiraga, S., & Donaldson, A. D. (2018). Rif1 acts through Protein Phosphatase 1 but independent of replication timing to suppress telomere extension in budding yeast. Nucleic Acids Research, 46(8), 3993–4003. https://doi.org/10.1093/nar/gky132