AmMag™ Quatro Mini-scale Plasmid, Antibody and Protein Purification

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The AmMag™ Quatro Mini-1100 is a fully automated plasmid and protein purification platform designed for mini-scale, high-throughput purification of plasmid DNA, proteins, and antibodies. The Quatro Mini-1100 system consists of the AmMag™ Quatro System Controller (D00055) and AmMag™ Quatro 1100 Automation Modules (D00043). A single System Controller can operate up to four independent purification modules simultaneously, providing a scalable and versatile solution for a wide range of laboratory workflows.

Featuring a dual-function design, each Automation Module can process up to 48 plasmid purification samples or 96 protein/antibody purification samples per run. A fully configured four-module system expands throughput to 192 plasmid samples or up to 384 protein/antibody samples in a single run. By combining flexible scalability with advanced magnetic bead automation, the AmMag™ Quatro Mini-1100 increases throughput, minimizes hands-on time, and delivers consistent, reproducible performance for genetic engineering, cell transfection, and protein expression applications.

Highly Efficient

~ 90% purity & recovery

Scalable

1-4 modules per controller

Automated

With Validated and Customizable Protocols

Dual functional

Plasmid DNA and protein/antibody

Dual-functional Specifics

	Plasmid Purification	Protein/Antibody Purification
Sample Types	High-copy and low-copy plasmid DNA	Cytosolic & secreted antibodies and proteins
Sample Type	E. Coli pellet	Culture
Plate format	48 deep-well	24 deep-well	48 deep-well	96 deep-well
Throughput	48 samples per module 192 samples with a 4-module setup	24 samples per module 96 samples with a 4-module setup	48 samples per module 192 samples with a 4-module setup	96 samples per module 384 samples with a 4-module setup
Sample Amount	≤ 2 mL	≤ 4 mL	≤ 2 mL	≤ 1 mL
Yield/Recovery	20 - 40 μg (high copy plasmid)	~ 90%
Total Time	< 90 minutes	< 60 minutes	< 60 minutes	< 90 minutes
Decontamination	UV light included	-
Endotoxin level	Low-endo kit < 1 EU/μg Endo-free kit < 0.1 EU/μg	-

Workflow

Figure 1. Workflow illustration of plasmid, antibody and protein purification on AmMag Quatro 1100.

Selection Guide

Automated Plasmid DNA Purification Solutions

	AmMag™ Quatro Mini - 1100	AmMag™ Quatro Maxi - 1400

Purification Method	Magnetic beads method based on alkaline lysis
Sample Type	E. Coli wet pellet with high/low copy plasmid
Sample Size	Wet pellet from 1-5 mL bacterial culture	Wet pellet mass 0.5-1.5 g (50-200 mL bacterial)
Throughput	Up to 48 samples per module Up to 192 samples with a 4-module setup	Up to 6 samples per module Up to 24 samples with a 4-module setup
Sample/Reaction Tube	AmMag™ 48 deep-well plate (provided)	AmMag™ Quatro 50 mL Tubes (provided)
Module	AmMag™ Quatro 1100 Automation Module	AmMag™ Quatro 1400 Automation Module
Controller	Mini Controller or Maxi Controller	Maxi Controller
Connectable Module Quant.	4 Automation Modules per Controller
Storage Condition	Room temperature

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Automated Antibody/Protein Purification Solutions

	AmMag™ Quatro Mini - 1100	AmMag™ Quatro ProAb - 1300

Purification Method	Magnetic beads based on affinity binding
Sample Type	Culture of mammalian cells, or wet pellet of E. Coli
Sample Size	Up to 1/2/4 mL culture	1 - 12 / 30 /65 mL culture
Throughput	24/48/96 samples per module 96/192/384 samples with a 4-module setup	12 samples
Sample/Reaction Tube	AmMag™ 24/48/96 deep-well plate	AmMag™ Quatro 25/50/100 mL tubes
Module	AmMag™ Quatro 1100 Automation Module	Attached
Controller	Mini Controller or Maxi Controller	Attached
Storage Condition	Room temperature

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Application Data

Case 1. AmMag™ Quatro Mini vs. Manual Kit for plasmid purification

Plasmid: pTT5. Strain: JM108. Culture medium: 2 mL TB. Culture time: 16 hours. Bacterial solution ODV: 17.

Sample	extraction method	Conc. (ng/µl)	Yield (µg)	OD260/280	OD260/230	Endotoxin (EU/µg)	Super coil (%)
1	Quatro 1100	279.7	31.6	1.88	2.30	0.1-0.2	96.2%
2	Manual	175.8	20.5	1.88	2.14	>200	95.4%

Concentration, yield, purity and supercoiling of the plasmid extracted by AmMag™ Quatro Mini are basically consistent with those of the manual kit. The endotoxin level islower, which meet the needs of downstream transfection applications.

Case 2. Homogeneity test in 48-well plate for plasmid purification

Plasmid: pcDNA3.1. Strain: DH5α. Culture medium: 2 mL TB. Culture time: 16 hours. Bacterial solution ODV: 16.

AmMag™ Quatro Mni module can complete 48 samples at one time, and the yield and supercoiling uniformity of samples are strong.
Case 3. Uniformity and functional test for antibody purification

Antibody: hIgG; Volume: 1 mL; Expression Host: CHO Titer: ~600 µg/mL.

The AmMag™ Quatro Mini 1100 can purify 48 samples per run, delivering > 80 % recovery for one elution, highly consistent yield with CV at 3%, and comparable bioactivity via ELISA compared to manual process and positive control.