AmMag™ Quatro ProAb 1300

Highlights

Fully Automated

From incubation to washing, elution, and regeneration

High-Throughput

Up to 12 samples with yields up to 320 mg per protein

Efficient

Purification in 30 minutes

Flexible

Customizable protocols for mini to midi scale

Traditional Resin vs. ProAb Purification Workflow

Product Offerings

AmMag™ Quatro ProAb Ordering Table

Recommended Magnetic Beads for Optimal Purification

Technical Data

• Updated Full-Auto Protein Purify System

  Improved Features	AmMag™ Quatro 1300 New	AmMag™ SA Discontinued
  Automation	Full automation with integrated mixing and liquid handling	Semi-automation
  Purification time	30 minutes, fully hands-off	45 minutes with manual sample transfers
  Max sample capacity	Up to 65 mL	Up to 50 mL
  Settled MagBeads volume per sample	Up to 6 mL	Up to 2 mL
  Buffer consumption	Optimized, reduced waste	High buffer consumption
  User interface	Simpler and user-friendly	Less intuitive

Instrument performance

• Consistent performance: Quatro ProAb 1300 vs Manual

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  Purity

  

  Activity

  

  Figure 1. The performance of AmMag™ Quatro ProAb 1300 Automated Purification System is consistent with that of the manual magnetic bead purification method.
  Take 30 mL of the fermentation broth of CHO cells overexpressing Anti-E2 hIgG, and use the Biolayer Interferometry (BLI) method to determine that the antibody titer is 923.3 μg/mL. Take 2 mL of GenScript Protein A Magnetic Beads (Cat# L00695), and use the AmMag™ Quatro ProAb 1300 Automated Purification System and the manual magnetic bead method respectively to purify the samples. Subsequently, conduct detection on the recovery rate, protein purity (SEC-HPLC), and binding activity of the purified antibodies.
  The results show that the recovery rates and purities (both > 95%) of the two purification methods are consistent, and the activities are both comparable to those of the positive control samples.
  PC: Positive control (sample purified by the protein purification media); GS-1300: Sample purified by GenScript AmMag™ Quatro 1300; GS-Manual: Sample purified by the manual method using GenScript magnetic beads (Cat# L00695).

• High Repeatability of Quatro ProAb 1300

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  Purity

  

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  Figure 2. The AmMag™ Quatro ProAb 1300 Automated Purification System demonstrates high repeatability.
  On the AmMag™ Quatro 1300 system, AmMag™ Quatro Elite Protein A Magnetic Beads 2G (Cat#L01074) were used to purify the supernatants of CHO cells overexpressing Anti-N protein hIgG (30 mL per sample). 12 same samples were purified in a single run and then the protein recovery rate, protein purity, and protein activity of the purified antibodies were measured.
  The results show that there is a high level of repeatability in the protein recovery rate, purity, and activity among the same 12 same samples in a single run, which proves that the AmMag™ Quatro 1300 Protein Purification System has high repeatability.
  PC: Positive control (sample purified by the protein purification media); GS-1300: Sample purified by GenScript AmMag™ Quatro 1300; GS-Manual: Sample purified by the manual method using GenScript magnetic beads (Cat#L01074).

Application

• Application: Different titer samples purified by Quatro 1300

  Samples information

  Sample type: hIgG; Expression System: CHO; Sample Volume: 30 mL; Titer: Sample 1: 923 μg/mL; Sample 2: 114μg/mL; Sample 3: 23 μg/mL; Beads Volume: 0.5 mL;

  Activity

  

  Sample 1

  

  Sample 2

  

  Sample 3

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  Purity

  

  Result: Quatro 1300 excels in purifying samples of varying titers with precision and reliability.