AmMag™ Quatro ProAb 1300

Automated Protein and Antibody Purification System

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Catalog Products » Protein Isolation and Purification » AmMag™ Quatro ProAb 1300 Automated Protein/Antibody Purification System

Overview

The AmMag™ Quatro ProAb 1300 provides high-purity, high-throughput magnetic protein and antibody purification for 12 samples in just 30 minutes. The ProAb 1300 fully automates bead incubation, washing, elution, and regeneration, minimizing manual intervention. The system is designed to accommodate mini to midi sample input scales, it efficiently processes up to 12 samples simultaneously. With six built-in liquid handling stations, ProAb offers flexible configuration to maximize throughput and operational efficiency.

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Highlights

Fully Automated

From incubation to washing, elution, and regeneration

High-Throughput

Up to 12 samples with yields up to 320 mg per protein

Efficient

Purification in 30 minutes

Flexible

Customizable protocols for mini to midi scale

Traditional Resin vs. ProAb Purification Workflow

Product Offerings

AmMag™ Quatro ProAb Ordering Table

Category	Cat. No.	Product Name	Qty.
Device	D00045	AmMag™ Quatro ProAb 1300 Automated Purification System	1 Unit
Consumables	D00061	AmMag™ 100 mL centrifuge tube	150 pcs/box
Consumables	D00060	AmMag™ Four-slot magnetic rod sleeve	30 pcs/box

Recommended Magnetic Beads for Optimal Purification

Category	Cat. No.	Magnetic Beads	Binding Capacity
Protein A beads	L00273	Protein A MagBeads	>10 mg Human IgG/mL settled beads
	L00695	AmMag™ Protein A Magnetic Beads	>40 mg Human IgG/mL settled beads
	L01071^New	AmMag™ Quatro Elite Protein A Magnetic Beads	>40 mg Human IgG/mL settled beads
	L01074^New	AmMag™ Quatro Elite Protein A Magnetic Beads, 2G	>35 mg Human IgG/mL settled beads
Protein G beads	L00274	Protein G MagBeads	>10 mg Human IgG/mL settled beads
Protein G beads	L00673	Protein G MagBeads MX	>30 mg Human IgG/mL settled beads
Nickel Beads	L00295	Ni-charged MagBeads	>40mg His-tagged Protein/mL settled beads
Nickel Beads	L00776	AmMag™ Ni Magnetic beads	>20mg His-tagged Protein/mL settled beads
GST Beads	L00895	Glutathione MagBeads	>20mg GST-tagged Protein/mL settled beads

Technical Data

Updated Full-Auto Protein Purify System

Improved Features	AmMag™ Quatro 1300^New	AmMag™ SA^Discontinued
Automation	Full automation with integrated mixing and liquid handling	Semi-automation
Purification time	30 minutes, fully hands-off	45 minutes with manual sample transfers
Max sample capacity	Up to 65 mL	Up to 50 mL
Settled MagBeads volume per sample	Up to 6 mL	Up to 2 mL
Buffer consumption	Optimized, reduced waste	High buffer consumption
User interface	Simpler and user-friendly	Less intuitive

Instrument performance

Methodology Consistency Validation

Consistent Performance between AmMag™ Quatro 1300 Automation and Conventional Manual Magnetic Bead Process.

Figure 1. Comparison of purification performance between the AmMag™ Quatro ProAb 1300 Automated System and manual magnetic bead purification. Using 30 mL of CHO cell fermentation broth overexpressing Anti-E2 hIgG (antibody titer 923.3 μg/mL by BLI), samples were purified with 2 mL GenScript Protein A Magnetic Beads (Cat# L00695) via both methods. Analysis of antibody recovery rate, protein purity (SEC-HPLC), and binding activity showed consistent results, with both methods achieving >95% purity and comparable activity to positive controls (PC: protein purification via media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification).
Intra-run repeatability Validation

High Repeatability of AmMag™ Quatro 1300 among 12 samples.

Figure 2. The AmMag™ Quatro ProAb 1300 Automated Purification System demonstrates high consistency across 12 samples processed simultaneously. Using AmMag™ Quatro Elite Protein A Magnetic Beads 2G (Cat# L01074), 12 identical 30 mL CHO cell supernatant samples overexpressing Anti-N protein hIgG were purified in a single run. Protein recovery rate, purity, and activity were measured and showed consistent results across all samples, validating the system’s within-run repeatability. (PC: Positive control purified by protein purification media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification with GenScript magnetic beads.)

Application

Sample Compatibility Validation

Exhibits broad compatibility with protein samples across low-to-high concentration ranges, rendering it suitable for both laboratory research and development (R&D) as well as early-stage process optimization.

Figure 3. The AmMag™ Quatro ProAb 1300 Automated Purification System demonstrates broad compatibility with protein samples across a wide concentration range. Using AmMag™ Quatro Elite Protein A Magnetic Beads 2G (Cat# L01074), 30 mL supernatants from three different CHO cell lines overexpressing hIgG with antibody titers ranging from 23 μg/mL to 923 μg/mL were purified. Protein recovery rate, purity, and activity measurements confirmed the system’s effective performance across low-to-high concentration samples, making it suitable for laboratory research, R&D, and early-stage process optimization. (PC: Positive control purified by protein purification media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification with GenScript magnetic beads.)