AmMag™ Quatro ProAb 1300

Highlights

Fully Automated

From incubation to washing, elution, and regeneration

High-Throughput

Up to 12 samples with yields up to 320 mg per protein

Efficient

Purification in 30 minutes

Flexible

Customizable protocols for mini to midi scale

Traditional Resin vs. ProAb 1300 Purification Workflow

Citations

Recommended Magnetic Beads for Optimal Purification

Learn More About Magnetic Beads >>

Selection Guide

	AmMag™ Quatro Mini - 1100	AmMag™ Quatro ProAb - 1300
Purification Method	Magnetic beads based on affinity binding
Sample Type	Culture of mammalian cells, or wet pellet of E. Coli
Sample Size	Up to 1/2/4 ml culture	1 - 12 / 30 /65 ml culture
Throughput	24/48/96 samples per module 96/192/384 samples with a 4-module setup	12 samples
Sample/Reaction Tube	AmMag™ 24/48/96 deep-well plate	AmMag™ Quatro 25/50/100 ml tubes
Automation Module	AmMag™ Quatro 1100 Automation Module	Attached
System Controller	Mini Controller or Maxi Controller
Storage Condition	Room temperature

Application Data

• Methodology Consistency Validation

  Consistent Performance between AmMag™ Quatro 1300 Automation and Conventional Manual Magnetic Bead Process.

  

  

  

  Figure 1. Comparison of purification performance between the AmMag™ Quatro ProAb 1300 Automated System and manual magnetic bead purification. Using 30 mL of CHO cell fermentation broth overexpressing Anti-E2 hIgG (antibody titer 923.3 μg/mL by BLI), samples were purified with 2 mL GenScript Protein A Magnetic Beads (Cat# L00695) via both methods. Analysis of antibody recovery rate, protein purity (SEC-HPLC), and binding activity showed consistent results, with both methods achieving >95% purity and comparable activity to positive controls (PC: protein purification via media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification).

• Intra-run repeatability Validation

  High Repeatability of AmMag™ Quatro 1300 among 12 samples.

  

  

  

  Figure 2. The AmMag™ Quatro ProAb 1300 Automated Purification System demonstrates high consistency across 12 samples processed simultaneously. Using AmMag™ Quatro Elite Protein A Magnetic Beads 2G (Cat# L01074), 12 identical 30 mL CHO cell supernatant samples overexpressing Anti-N protein hIgG were purified in a single run. Protein recovery rate, purity, and activity were measured and showed consistent results across all samples, validating the system’s within-run repeatability. (PC: Positive control purified by protein purification media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification with GenScript magnetic beads.)

• Sample Compatibility Validation

  Exhibits broad compatibility with protein samples across low-to-high concentration ranges, rendering it suitable for both laboratory research and development (R&D) as well as early-stage process optimization.

  

  

  

  

  

  Figure 3. The AmMag™ Quatro ProAb 1300 Automated Purification System demonstrates broad compatibility with protein samples across a wide concentration range. Using AmMag™ Quatro Elite Protein A Magnetic Beads 2G (Cat# L01074), 30 mL supernatants from three different CHO cell lines overexpressing hIgG with antibody titers ranging from 23 μg/mL to 923 μg/mL were purified. Protein recovery rate, purity, and activity measurements confirmed the system’s effective performance across low-to-high concentration samples, making it suitable for laboratory research, R&D, and early-stage process optimization. (PC: Positive control purified by protein purification media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification with GenScript magnetic beads.)

• Protocol: Cell Lysis for His-tagged Protein Purification using the AmMag™ Quatro ProAb 1300
  

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