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Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » c-Myc-tag Antibody, pAb, Rabbit

c-Myc-tag Antibody, pAb, Rabbit

C-Myc-tag Antibody, PAb, Rabbit

Western blot analysis of c-Myc fusion protein (MW≈52KD) using 1 µg/ml Rabbit Anti-c-Myc-tag Polyclonal Antibody (GenScript, A00172)
Lane 1-3: c-Myc fusion protein in HEK293 cell lysate
Lane 4: Negative HEK293 cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

C-Myc-tag Antibody, PAb, Rabbit

Western blot analysis of c-Myc tagged fusion proteins expressed in E. coli cell lysate using c-Myc-tag Antibody (GenScript, A00172, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

C-Myc-tag Antibody, PAb, Rabbit

Customer Reviews:
c-Myc-tag Antibody, pAb, Rabbit (GenScript,A00172) were used in western blotting to show overexpression of Bik1, Pac1, or Dyn1 by GAL1 promoter in budding yeast.
Left and middle:
Western blot of cell lysates prepared from strains with either endogenous or GAL1 promoter driving expression of indicated 13myc-tagged gene.
Right:
Western blot of Dyn1-13myc protein in strains with either the endogenous or GAL1 promoter driving the expression of PAC1 or BIK1.
For all blots, equal amount of total cell lysate was loaded in each lane, transferred to PVDF and probed with anti-c-Myc antibody(GenScript, A00172).
All strains were grown to mid-log phase in SD media supplemented with 2% galactose, and then harvested.

C-Myc-tag Antibody, PAb, Rabbit

Western blot analysis of immunoprecipitates from c-Myc-tagged protein using c-Myc-tag Antibody, pAb, Rabbit (Cat. No. A00172).
Lane 1: c-Myc tagged fusion protein as control.
Lane 2: Immunoprecipitates of c-Myc tagged fusion protein incubated with Rabbit IgG Control (GenScript, Cat. No. A01008) and Protein A served as negative control
Lane 3: Immunoprecipitates of c-Myc tagged fusion protein incubated with c-Myc-tag Antibody, pAb, Rabbit (GenScript, A00172) and Protein A.

The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Rabbit IgG.

Specificity This Antibody recognizes c-Myc tagged fusion proteins.
Host Species Rabbit
Immunogen c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Immunofluorescence 5-20 µg/ml
Immunoprecipitation (IP) 2-5 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

  • C-Myc-tag Antibody, PAb, Rabbit
  • C-Myc-tag Antibody, PAb, Rabbit

    Western blot analysis of c-Myc fusion protein (MW≈52KD) using 1 µg/ml Rabbit Anti-c-Myc-tag Polyclonal Antibody (GenScript, A00172)
    Lane 1-3: c-Myc fusion protein in HEK293 cell lysate
    Lane 4: Negative HEK293 cell lysate
    Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

  • C-Myc-tag Antibody, PAb, Rabbit
  • C-Myc-tag Antibody, PAb, Rabbit

    Western blot analysis of c-Myc tagged fusion proteins expressed in E. coli cell lysate using c-Myc-tag Antibody (GenScript, A00172, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

  • C-Myc-tag Antibody, PAb, Rabbit
  • C-Myc-tag Antibody, PAb, Rabbit

    Customer Reviews:
    c-Myc-tag Antibody, pAb, Rabbit (GenScript,A00172) were used in western blotting to show overexpression of Bik1, Pac1, or Dyn1 by GAL1 promoter in budding yeast.
    Left and middle:
    Western blot of cell lysates prepared from strains with either endogenous or GAL1 promoter driving expression of indicated 13myc-tagged gene.
    Right:
    Western blot of Dyn1-13myc protein in strains with either the endogenous or GAL1 promoter driving the expression of PAC1 or BIK1.
    For all blots, equal amount of total cell lysate was loaded in each lane, transferred to PVDF and probed with anti-c-Myc antibody(GenScript, A00172).
    All strains were grown to mid-log phase in SD media supplemented with 2% galactose, and then harvested.

  • C-Myc-tag Antibody, PAb, Rabbit
  • C-Myc-tag Antibody, PAb, Rabbit

    Western blot analysis of immunoprecipitates from c-Myc-tagged protein using c-Myc-tag Antibody, pAb, Rabbit (Cat. No. A00172).
    Lane 1: c-Myc tagged fusion protein as control.
    Lane 2: Immunoprecipitates of c-Myc tagged fusion protein incubated with Rabbit IgG Control (GenScript, Cat. No. A01008) and Protein A served as negative control
    Lane 3: Immunoprecipitates of c-Myc tagged fusion protein incubated with c-Myc-tag Antibody, pAb, Rabbit (GenScript, A00172) and Protein A.

    The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Rabbit IgG.


Target Background The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24.c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen.c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins.This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Synonyms Rabbit Anti-c-Myc-tag pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Cat. No. Product Name Quantity Price
A00864-200
THE™ c-Myc Antibody [Biotin], mAb, Mouse - 200 μg
$333.00
A00863-100
THE™ c-Myc Antibody [HRP], mAb, Mouse - 100 μg
$180.00
A00704-100
THE™ c-Myc Tag Antibody, mAb, Mouse - 100 μg
$175.00
A00173-40
c-Myc-tag Antibody [HRP], pAb, Rabbit - 40 μg
$99.00
A00172-40
c-Myc-tag Antibody, pAb, Rabbit - 40 μg
$99.00
 
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XuQian, et al. Domain-specific expression of meristematic genes is defined by the LITTLE ZIPPER protein DTM in tomato. Commun Biol. (2019-01)

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Wu JL, et al. O-GlcNAcylation is required for B cell homeostasis and antibody responses. Nat Commun. (2017)

Wang Y, et al. The Auxiliary Calcium Channel Subunit α2δ4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors. Neuron. (2017)

LiuDong, et al. SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity. Mol. Cell. Biol. (2015)

Xu K, et al. Sperm-specific AKAP3 is a dual specificity anchoring protien that interacts with both protein kinase A regulatory subunits via conserved N-terminal amphipathic peptides. Mol Reprod Dev. (2014-03)

D Liu, et al. eEF1Bγ is a positive regulator of NF-κB signaling pathway. Biochem Biophys Res Commun. (2014-03)

Shuai Chen, et al. Enhancer of Zeste Homolog 2 Is a Negative Regulator of Mitochondria-Mediated Innate Immune Responses. J Immunol. (2013-8)

Orlandi C, et al. Orphan receptor GPR179 forms macromolecular complexes with components of metabotropic signaling cascade in retina ON-bipolar neurons. Invest Ophthalmol Vis Sci. (2013)

Zhang B, et al. The p16-Specific Reactivation and Inhibition of Cell Migration Through Demethylation of CpG Islands by Engineered Transcription Factors. Hum Gene Ther. (2012-10)

James J. Matsuda, et al. Activation of IClswell by TNF-α requires CIC-3-dependent endosomal reactive oxygen production. J Biol Chem. (2010-07)

Jay H. Lo and Thomas T. Chen., et al. CCAAT/Enhancer Binding Protein β2 Is Involved in Growth Hormone-Regulated Insulin-Like Growth Factor-II Gene Expression in the Liver of Rainbow Trout (Oncorhynchus mykiss). Endocrinology. (2010-05)

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Cat. No. A00172
Size Price
40 μg $99.00