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Catalog Products » Antibodies » THE Elite Antibodies » THE™ HA-tag mAb » His-tag Antibody, pAb, Rabbit

His-tag Antibody, pAb, Rabbit

Specificity GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags.For N-terminal His-tagged protein detection, THE™ Anti-His mAb (Cat.No.A00186) is recommended.
Host Species Rabbit
Immunogen His epitope tag peptide HHHHHH conjugated to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Target Background His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells.Short-sequence epitope tags are common in the study of protein expression in several different expression systems.In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.
Synonyms His-tag Antibody, pAb, Rabbit;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Cat. No. Product Name Quantity Price
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
Anti-His Affinity Resin - 1 ml Resin (Total 2 ml)
Anti-His Affinity Resin - 5 ml Resin (Total 10 ml)
His Tag ELISA Detection Kit - 1 kit
THE™ His Tag Antibody [iFluor 647], mAb, Mouse - 100 μg
THE™ His Tag Antibody [iFluor 555], mAb, Mouse - 100 μg
THE™ His Tag Antibody [iFluor 488], mAb, Mouse - 100 μg
THE™ His Tag Antibody [FITC], mAb, Mouse - 100 μg
THE™ His Tag Antibody [Biotin], mAb, Mouse - 100 μg
THE™ His Tag Antibody [HRP], mAb, Mouse - 100 μg
His-tag antibody [Biotin], pAb, Rabbit - 40 μg
THE™ His Tag Antibody, mAb, Mouse - 100 μg
His-tag Antibody, pAb, Rabbit - 40 μg
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Cat. No. A00174
Size Price
40 μg $99.00