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Catalog Products » Antibodies » Epitope Tag Antibodies » Avi-tag Antibody, pAb, Rabbit

Avi-tag Antibody, pAb, Rabbit

Avi-tag Antibody, PAb, Rabbit

Loading: Avi-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 ug/ml Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204C)

Avi-tag Antibody, PAb, Rabbit

Western blot analysis of Avi tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-Avi-tag pAb; Rabbit Anti-Avi-tag
Description Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. GenScript Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Avi tag peptide conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity This Antibody recognizes Avi tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Avi-tag Antibody, PAb, Rabbit
Avi-tag Antibody, PAb, Rabbit

Loading: Avi-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 ug/ml Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204C)

Avi-tag Antibody, PAb, Rabbit
Avi-tag Antibody, PAb, Rabbit

Western blot analysis of Avi tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


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Cat. No. A00674
Size
Price
40 ug $99.00
 
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