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Catalog Products » Antibodies » THE Elite Antibodies » THE™ HA-tag mAb » Avi-tag Antibody, pAb, Rabbit

Avi-tag Antibody, pAb, Rabbit

Avi-tag Antibody, PAb, Rabbit

Loading: Avi-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 ug/ml Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204C)

Avi-tag Antibody, PAb, Rabbit

Western blot analysis of Avi tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Specificity This Antibody recognizes Avi tagged fusion proteins.
Host Species Rabbit
Immunogen Avi tag peptide conjugated to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

  • Avi-tag Antibody, PAb, Rabbit
  • Avi-tag Antibody, PAb, Rabbit

    Loading: Avi-tag fusion protein expressed in E. coli cell lysate
    Antibody: 1 ug/ml Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674)
    The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204C)

  • Avi-tag Antibody, PAb, Rabbit
  • Avi-tag Antibody, PAb, Rabbit

    Western blot analysis of Avi tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Target Background Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE.The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.GenScript Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
Synonyms Rabbit Anti-Avi-tag pAb;Rabbit Anti-Avi-tag

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Cat. No. Product Name Quantity Price
A01738-40
Avi Tag Antibody, mAb, Mouse - 40 μg
$99.00
 
Tyler N Starr, et al. SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape. Nature. (2021-07)

Mayara Garcia de Mattos Barbosa, et al. IgV somatic mutation of human anti-SARS-CoV-2 monoclonal antibodies governs neutralization and breadth of reactivity. JCI Insight. (2021-05)

SheridanRyan M, et al. Widespread Backtracking by RNA Pol II Is a Major Effector of Gene Activation, 5' Pause Release, Termination, and Transcription Elongation Rate. Mol Cell. (2019-01)

GalvanAdri M, et al. Cell-associated heparin-like molecules modulate the ability of LDL to regulate PCSK9 uptake. J. Lipid Res. (2019-01)

Cortazar MA1, et al. Control of RNA Pol II Speed by PNUTS-PP1 and Spt5 Dephosphorylation Facilitates Termination by a "Sitting Duck Torpedo" Mechanism. Mol Cell. (2019)

Chin S Michael, et al. Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab. Nat Commun. (2018)

Chen XQ, et al. T-complex protein 1-ring complex enhances retrograde axonal transport by modulating tau phosphorylation. Traffic. (2018)

X Zhao, et al. Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons. J Vis Exp. (2014-09)

Wicht O, et al. Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell. J Virol. (2014-02)

JD Stender, et al. Control Of Proinflammatory Gene Programs By Regulated Trimethylation And Demethylation Of Histone H4K20. Mol Cell. (2012-12)

Sung K, et al. A novel method for producing mono-biotinylated, biologically active neurotrophic factors: An essential reagent for single molecule study of axonal transport. J Neurosci Methods. (2011-09)

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For more documents, please visit "Technical Support".

Cat. No. A00674
Size Price
40 μg $99.00