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Avi-tag Antibody, PAb, Rabbit

Loading: Avi-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 ug/ml Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204C)

Avi-tag Antibody, PAb, Rabbit

Western blot analysis of Avi tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Avi-tag Antibody, pAb, Rabbit

This Antibody recognizes Avi tagged fusion proteins.
A00674
$99.00

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Overview
Specificity This Antibody recognizes Avi tagged fusion proteins.
Host Species Rabbit
Immunogen Avi tag peptide conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Examples
  • Avi-tag Antibody, PAb, Rabbit
  • Avi-tag Antibody, PAb, Rabbit

    Loading: Avi-tag fusion protein expressed in E. coli cell lysate
    Antibody: 1 ug/ml Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674)
    The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204C)

  • Avi-tag Antibody, PAb, Rabbit
  • Avi-tag Antibody, PAb, Rabbit

    Western blot analysis of Avi tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Avi-tag Polyclonal Antibody (GenScript, A00674, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Background
Target Background Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE.The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.GenScript Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
Synonyms Rabbit Anti-Avi-tag pAb;Rabbit Anti-Avi-tag

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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