Catalog Products » Antibodies » THE Elite Antibodies » THE™ PEG Antibody, mAb, Mouse
THE™ PEG Antibody, MAb, Mouse

Figure 1. Double-antigen sandwich ELISA analysis of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795). PEG antibody was captured by PEG40K-OVA and detected by PEG20K-Biotin and Streptavidin-HRP conjugate. The test result shows that GenScript’s PEG Antibody is suitable for Bridging ELISA application.

THE™ PEG Antibody, MAb, Mouse

Figure 2. Immunohistochemistry analysis of mouse kidney tissue (Paraffin embedded) using THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; 10 µg/mL) after mice were injected with BSA-PEG as an immunogen. The test results show that GenScript’s PEG Antibody is suitable for IHC application.

THE™ PEG Antibody, MAb, Mouse

Figure 3. In-Cell ELISA analysis of PEGylated drug-HepG2 interaction using THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; 1 µg/mL) after HepG2 cells were treated with PEGylated drug (PEGASYS®, Peginterferon alfa-2a) at different concentrations. The test results show that GenScript’s PEG Antibody is suitable for the analysis of PEGylated drug-cell interaction.

THE™ PEG Antibody, MAb, Mouse

Figure 4. Sensitivity comparison of competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4 ) with THE™ PEG Antibody, mAb, Mouse (GenScript, A01795) by Western Blot.

A,C: PEGylated drug (PEGASYS®, Peginterferon alfa-2a)
B,D: Interferon Alfa 2A protein

In both panels A and B, the primary antibody of was Mouse Anti-PEG mAb (Company A, Clone AGP4) used at 0.2 µg/mL concentration;
In both panels C and D, the primary antibody of was THE™ PEG Antibody, mAb, Mouse (GenScript, A01795) used at 0.2 µg/mL concentration;

The test results show that GenScript’s PEG Antibody has better sensitivity and specificity than the competitor’s product.

THE™ PEG Antibody, MAb, Mouse

Figure 5. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red), and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; black) by indirect ELISA, with plate coated with PEG40K-OVA. The test results show that GenScript’s PEG Antibody had better reactivity to PEG40K-OVA than the competitor’s product.

THE™ PEG Antibody, MAb, Mouse

Figure 6. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; blue), and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; red) by indirect ELISA. The test results show that GenScript’s PEG Antibody could detect PEG with different molecular weights and has better reactivity to PEG40K, PEG20K, PEG5K and PEG12 (Pierce, MES(PEG)12) than the competitor’s product.

THE™ PEG Antibody, MAb, Mouse

Figure 7. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red) and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; yellow) by indirect ELISA. The test results show that GenScript’s PEG Antibody had better reactivity to PEG20K than the competitor’s product.

THE™ PEG Antibody, MAb, Mouse

Figure 8. The half maximal inhibitory concentration (IC50) comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red) and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; black) in competitive ELISA. The ELISA plate was coated with PEG40K-OVA, and unconjugated PEG40K was used for determining the IC50 of anti-PEG mAb. The test results showed that GenScript’s PEG Antibody had lower IC50, therefore, better specificity and higher affinity to PEG were validated.

THE™ PEG Antibody, mAb, Mouse

GenScript’s THE™ PEG Antibody, mAb, Mouse has high affinity for the PEG backbone. The antibody binds to the variety of PEG such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates. 
A01795
$144.00

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Overview
Specificity GenScript’s THE™ PEG Antibody, mAb, Mouse has high affinity for the PEG backbone. The antibody binds to the variety of PEG such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates. 
Host Species Mouse
Immunogen PEG conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
Application Recommended Usage
Sandwich ELISA Capture: 5-10 µg/ml
Detection: 0.1-1.0 µg/ml
Competitive ELISA User-optimized
Western Blot 0.1-1.0 µg/ml
Immunohistochemistry (IHC) 10-15 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. For long-term storage, adding glycerol to a final concentration of 50% (v/v) in the reconstituted antibody solution is recommended. 
Storage Instructions The lyophilized product remains stable for up to 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles. 
Purification Affinity chromatography
Isotype Mouse IgM
Clonality Monoclonal
Clone ID 5E10E9
Note Some reagents such as Tween-20, Triton X-100 and NP-40 interfere with the PEG antibody interactions due to their structural similarity to PEG. Antibody solution should not contain such reagents in the assay of PEGylated molecules.
GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 1. Double-antigen sandwich ELISA analysis of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795). PEG antibody was captured by PEG40K-OVA and detected by PEG20K-Biotin and Streptavidin-HRP conjugate. The test result shows that GenScript’s PEG Antibody is suitable for Bridging ELISA application.

  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 2. Immunohistochemistry analysis of mouse kidney tissue (Paraffin embedded) using THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; 10 µg/mL) after mice were injected with BSA-PEG as an immunogen. The test results show that GenScript’s PEG Antibody is suitable for IHC application.

  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 3. In-Cell ELISA analysis of PEGylated drug-HepG2 interaction using THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; 1 µg/mL) after HepG2 cells were treated with PEGylated drug (PEGASYS®, Peginterferon alfa-2a) at different concentrations. The test results show that GenScript’s PEG Antibody is suitable for the analysis of PEGylated drug-cell interaction.

  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 4. Sensitivity comparison of competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4 ) with THE™ PEG Antibody, mAb, Mouse (GenScript, A01795) by Western Blot.

    A,C: PEGylated drug (PEGASYS®, Peginterferon alfa-2a)
    B,D: Interferon Alfa 2A protein

    In both panels A and B, the primary antibody of was Mouse Anti-PEG mAb (Company A, Clone AGP4) used at 0.2 µg/mL concentration;
    In both panels C and D, the primary antibody of was THE™ PEG Antibody, mAb, Mouse (GenScript, A01795) used at 0.2 µg/mL concentration;

    The test results show that GenScript’s PEG Antibody has better sensitivity and specificity than the competitor’s product.

  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 5. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red), and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; black) by indirect ELISA, with plate coated with PEG40K-OVA. The test results show that GenScript’s PEG Antibody had better reactivity to PEG40K-OVA than the competitor’s product.

  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 6. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; blue), and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; red) by indirect ELISA. The test results show that GenScript’s PEG Antibody could detect PEG with different molecular weights and has better reactivity to PEG40K, PEG20K, PEG5K and PEG12 (Pierce, MES(PEG)12) than the competitor’s product.

  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 7. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red) and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; yellow) by indirect ELISA. The test results show that GenScript’s PEG Antibody had better reactivity to PEG20K than the competitor’s product.

  • THE™ PEG Antibody, MAb, Mouse
  • THE™ PEG Antibody, MAb, Mouse

    Figure 8. The half maximal inhibitory concentration (IC50) comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red) and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; black) in competitive ELISA. The ELISA plate was coated with PEG40K-OVA, and unconjugated PEG40K was used for determining the IC50 of anti-PEG mAb. The test results showed that GenScript’s PEG Antibody had lower IC50, therefore, better specificity and higher affinity to PEG were validated.


Background
Target Background PEG (Polyethylene glycol) is a polyether compound with many applications from industrial manufacturing to medicine. PEGylation is a technology that covalently couples non-toxic, hydrophilic polyethylene glycol (PEG) to the drug. It is an FDA-approved method for the delivery of protein drugs. PEG modification can reduce the drug immunogenicity and antigenicity. PEGylated drug decelerates renal excretion, improves stability towards proteolysis and increases its half-life in blood. Accurate and sensitive quantification of PEG conjugates is important for PEG conjugated product development and pharmaceutical study. Polyethylene glycol (PEG) antibody is a useful tool for the detection of PEGylated molecules.
Synonyms Anti-Polyethylene glycol antibody, mAb, Mouse

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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