The primer extension assay to detection the blocking activity of Hot Start Taq Antibody. All groups were added with 1.25 U Taq DNA polymerase pretreated with 0, 0.25, 0.5, 1, 2 U (Lane 1-5) Hot Start Taq Antibody.
The PCR specificity detection of Hot Start Taq Antibody. The Antibody used in each group is 2, 1, 0.5, 0 U (lane 1-2, 3-4, 5-6, 7-8).
Synonyms | Taq Antibody |
Description | The Hot Start Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling. |
Host Species | Mouse |
Conjugation | Unconjugated |
Immunogen | Taq DNA polymerase |
Concentration | 5 U/μl |
Specificity | Taq DNA polymerase |
Storage | The recommended storage temperature is -20°C. Aliquot to avoid repeated freeze/thaw cycles. |
Note | One unit is defined as the amount of antibody required to inhibit greater than 90% one unit of Taq DNA polymerase after a 10 minute incubation at 55°C. |
Hot Start PCR Western Blot Assay |
The primer extension assay to detection the blocking activity of Hot Start Taq Antibody. All groups were added with 1.25 U Taq DNA polymerase pretreated with 0, 0.25, 0.5, 1, 2 U (Lane 1-5) Hot Start Taq Antibody.
The PCR specificity detection of Hot Start Taq Antibody. The Antibody used in each group is 2, 1, 0.5, 0 U (lane 1-2, 3-4, 5-6, 7-8).
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