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Hot Start Taq Antibody

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The primer extension assay to detection the blocking activity of Hot Start Taq Antibody. All groups were added with 1.25 U Taq DNA polymerase pretreated with 0, 0.25, 0.5, 1, 2 U (Lane 1-5) Hot Start Taq Antibody.

The PCR specificity detection of Hot Start Taq Antibody. The Antibody used in each group is 2, 1, 0.5, 0 U (lane 1-2, 3-4, 5-6, 7-8).

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Overview

Synonyms	Taq Antibody
Description	The Hot Start Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling.
Host Species	mouse
Conjugation	Unconjugated
Immunogen	Taq DNA polymerase

Properties

Concentration	5 U/μl
Specificity	Taq DNA polymerase
Storage	The recommended storage temperature is -20°C. Aliquot to avoid repeated freeze/thaw cycles.
Note	One unit is defined as the amount of antibody required to inhibit greater than 90% one unit of Taq DNA polymerase after a 10 minute incubation at 55°C.