Hot Start Taq Antibody
The primer extension assay to detection the blocking activity of Hot Start Taq Antibody. All groups were added with 1.25 U Taq DNA polymerase pretreated with 0, 0.25, 0.5, 1, 2 U (Lane 1-5) Hot Start Taq Antibody.
The PCR specificity detection of Hot Start Taq Antibody. The Antibody used in each group is 2, 1, 0.5, 0 U (lane 1-2, 3-4, 5-6, 7-8).
| Synonyms | Taq Antibody |
| Description | The Hot Start Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | Taq DNA polymerase |
| Concentration | 5 U/μl |
| Specificity | Taq DNA polymerase |
| Storage | The recommended storage temperature is -20°C. Aliquot to avoid repeated freeze/thaw cycles. |
| Note | One unit is defined as the amount of antibody required to inhibit greater than 90% one unit of Taq DNA polymerase after a 10 minute incubation at 55°C. |
| Hot Start PCR Western Blot Assay |
The primer extension assay to detection the blocking activity of Hot Start Taq Antibody. All groups were added with 1.25 U Taq DNA polymerase pretreated with 0, 0.25, 0.5, 1, 2 U (Lane 1-5) Hot Start Taq Antibody.
The PCR specificity detection of Hot Start Taq Antibody. The Antibody used in each group is 2, 1, 0.5, 0 U (lane 1-2, 3-4, 5-6, 7-8).
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| Cat. No. A01849 | ||
|---|---|---|
| Size | Price | |
| 250 U (5U/μl) | $120.00 | |
| 1000 U (5U/μl) | $380.00 | |
