eStain™ L1 Protein Staining System
10-min Protein Gel Staining with ultrasensitivity and simplicity
- Versatile: Gel staining with Coomasie Blue (offered by GenScript) or membrane staining with Ponceau S solution
- Fast: Stain and destain in less than 10 min
- Automatic: Reagents are pumped in/out automatically
- Sensitive: sharply stained protein bands and sensitivity ≥ 12.5 ng
- Consistent: Results are highly repeatable
- Efficient: Simultaneously stain/destain two mini gels or membranes
eStain is a highly efficient protein PAGE gel staining system, which uses Coomassie Brilliant Blue and a patented protein staining technology developed by Genscript. eStain staining system integrates the traditional three steps of fixing-staining-destaining into one and can stain/destain two protein PAGE gels simultaneously in 10 minutes or less.
eStain works with all types of precast as well as homemade PAGE gels. Compared with the conventional staining methods, gels stained by eStain have crisp blue bands with minimum or no background. eStain also offers superior sensitivity compared to conventional methods and can detect as low as 12.5 ng of protein.
| eStain Videos |
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Introductory Video
| eStain™ L1 Resources |
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Shortened workflow increases your productivity
The eStain Protein Staining System combines three steps of traditional Coomassie blue staining method into one single step to greatly reduce the time required for protein staining (Table 1).
Table 1: eStain saves hours to overnight compared to conventional staining methods
| Wash or Rinse Step | Stain Step | Destain or Rinse Step | Time saved with eStain L1 | |
|---|---|---|---|---|
| eStain | 0 min | Electric Staining <10 min | 0 min | |
| SimplyBlue SafeSatin (Basic Protocol) | Rinse 15 min | Stain 1-3 hr | Wash 1-3 hr | 2 hr , 15 min-6 hr, 15 min |
| Bio-Safe Coomassie Stain | Wash 15 min | Stain 1 hr | Rinse 30 min | 1 hr , 45 min |
| Gelcode Blue Safe Protein Stain (Basic Protocol) | Wash 15 min | Stain 15 min-1 hr | Destain 1-2 hr | 1 hr , 30 min - 3 hr , 15 min |
Excellent uniformity and repeatability with either Coomasie Blue and Ponceau S solution
- Gel: SurePAGE™ Gel, Bis-Tris, 4-12% (GenScript, M00653)
- Membrane: GenScript nitrocellulose, PVDF membranes
- Samples: Pre-stained protein standard (GenScript, M006245); unstained PAGE-Master protein standard (GenScript, M00516)
- Program: Stain
- Staining Solution: Ponceau S solution
- Time: 9 min 30 s
- Gel: ExpressPlus™ PAGE Gel, 4-12%, 10 wells (GenScript M41210)
- Samples: PAGE-MASTER Protein Standard Plus, 5 μl (GenScript, MM1397-500)
- Program: Stain
- Staining solution: Coomasie Blue
- Time: 9 min 30 s
Superior sensitivity (≥12.5 ng protein)
- Gel: ExpressPlus™ PAGE Gel, 4-12%, 10 wells (GenScript M41210)
- Program: Stain
- Time: 9 min 30 s
eStain Protein Staining device quick guide
Note: For complete instructions and advanced programming, please refer to the manual
| Operational Instructions | |
|---|---|
| Step 1. Add distilled water into the tray provided. When gel electrophoresis is complete, carefully remove the gel from the gel cassette and briefly rinse the gel with distilled water for 1 min |  |
| Step 2. Open the gel holder and place it on the table with the fabric side down. Carefully place the rinsed gel on the fabric side of the gel holder. Ensure that the upper part of the gel (with comb) point to the axis of the gel holder and is as close to the axis as possible |  |
| Step 3. Pre-wet a piece of filter paper (provided) with distilled water and place it on top of the gel | |
| Step 4. Close the gel holder | |
| Step 5. Choose a Channel and insert the gel holder into the channel.
Note：Please ensure that the fabric side of the gel holder is facing the user before inserting it into the Channel |
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| Step 6. Press the corresponding channel to start the program. The channel timer starts to countdown |  |
| Step 7. The instrument beeps as the program countdown to 0 |  |
| Step 8. The channel stops flashing and the screen returns to normal. Take out the gel holder and the stained gel. The staining process is complete. | |
Reagent Consumables
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00753 |
eStain L1C Protein Staining Kit - 1 Unit |
$102.00 |
Instrument Consumables
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00662-20 |
Tube - 20 m |
$99.00 |
|
L00662-10 |
Tube - 10 m |
$69.00 |
|
L00661-5 |
Liquid container - 1 unit (5L) |
$39.00 |
|
L00661-10 |
Liquid container - 1 unit (10L) |
$49.00 |
|
L00663 |
Small Two Pass Screw Cap(Fit 5L and 10L container) - 3 pk |
$69.00 |
|
L00658 |
eStain Gel Holder - 2 unit |
$349.00 |
eStain Protein Staining Device
| Cat. No. | Product Name | Size | Price | Quantity |
|---|---|---|---|---|
| L00657 | eStain Protein Staining Device | 1 unit | Quote | 1 |
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*Promotion applies to US and Canada customers only.
*Promotion cannot be combined with any other existing offers, discounts, or coupons.
