Catalog Products » Protein Isolation and Analysis » eStain^® L1 Protein Staining System

eStain^® L1 Protein Staining System

10-min Protein Gel Staining with ultrasensitivity and simplicity

Fast: stain/destain in 10 min or less
Automatic: reagents are pumped in/out automatically
Sensitive: sharply stained protein bands and sensitivity ≥ 12.5ng
Efficient: simultaneously stain/destain two mini gels
Reliable: evenly stain and highly repeatable results

eStain L1 is a highly efficient protein PAGE gel staining system, which uses Coomassie Brilliant Blue and a patented protein staining technology developed by Genscript. eStain L1 staining system integrates the traditional three steps of fixing-staining-destaining into one and can stain/destain two protein PAGE gels simultaneously in 10 minutes or less.

eStain L1 works with all types of precast as well as homemade PAGE gels. Compared with the conventional staining methods, gels stained by eStain L1 have crisp blue bands with minimum or no background. eStain L1 also offers superior sensitivity compared to conventional methods and can detect as low as 12.5 ng of protein.

eStain L1 videos
Introductory video Detailed set up and operation video

eStain^® L1 Resources
eStain L1 User Manual estain L1 Quick Reference Card eStain L1 Flyer

Why choose eStain^® L1

Shortened workflow increases your productivity

The eStain L1 Protein Staining System combines three steps of traditional Coomassie blue staining method into one single step to greatly reduce the time required for protein staining (Table 1).

Table 1: eStain L1 saves hours to overnight compared to conventional staining methods

	wash or Rinse Step	Stain Step	Destain or Rinse Step	Time saved with eStain™ L1
eStain L1	0 min	Electric Staining <10 min	0 min
SimplyBlue™ SafeSatin (Basic Protocol)	Rinse 15 min	Stain 1-3 hr	Wash 1-3 hr	2 hr , 15 min-6 hr, 15 min
Bio-Safe Coomassie Stain	Wash 15 min	Stain 1 hr	Rinse 30 min	1 hr , 45 min
Gelcode™ Blue Safe Protein Stain (Basic Protocol)	Wash 15 min	Stain 15 min-1 hr	Destain 1-2 hr	1 hr , 30 min - 3 hr , 15 min

Superior sensitivity (≥12.5ng protein)

Gel: ExpressPlus PAGE Gel, 4-12%, 10 wells (GenScript M41210)
Program: Stain
Time: 9 min 30 s

Excellent uniformity and repeatability

Gel: ExpressPlus PAGE Gel, 4-12%, 10 wells (GenScript, M41210)
Samples: PAGE-MASTER Protein Standard Plus, 5 ul (GenScript, MM1397-500)
Program: Stain
Time: 9 min 30 s

Quick Procedures

eStain L1 Protein Staining device quick guide

Note: For complete instructions and advanced programming, please refer to the manual

Operational Instructions
Step 1. Add distilled water into the tray provided. When gel electrophoresis is complete, carefully remove the gel from the gel cassette and briefly rinse the gel with distilled water for 1 min
Step 2. Open the gel holder and place it on the table with the fabric side down. Carefully place the rinsed gel on the fabric side of the gel holder. Ensure that the upper part of the gel (with comb) point to the axis of the gel holder and is as close to the axis as possible
Step 3. Pre-wet a piece of filter paper (provided) with distilled water and place it on top of the gel
Step 4. Close the gel holder
Step 5. Choose a Channel and insert the gel holder into the channel. *Note：Please ensure that the fabric side of the gel holder is facing the user before inserting it into the Channel*
Step 6. Press the corresponding channel to start the program. The channel timer starts to countdown
Step 7. The instrument beeps as the program countdown to 0
Step 8. The channel stops flashing and the screen returns to normal. Take out the gel holder and the stained gel. The staining process is complete.

Consumables Ordering

Reagent Consumables

	Cat. No.	Product Name	Price
	L00659-1	eStain L1 Protein Staining Kit - 1 unit	$160.00
	L00753	eStain L1C Protein Staining Kit - 1 Unit	$99.00

Instrument Consumables

Cat. No.	Product Name	Price
L00662-20	eStain L1 Tube - 20 m	$99.00
L00662-10	eStain L1 Tube - 10 m	$69.00
L00661-5	eStain L1 Liquid container - 1 unit	$39.00
L00661-10	eStain L1 Liquid container - 1 unit	$49.00
L00663	eStain L1 Small Two Pass Screw Cap(Fit 5L and 10L container) - 1 unit	$69.00
L00658	eStain L1 Gel Holder - 2 unit	$349.00

Device Ordering

eStain L1 Protein Staining Device

Cat. No.	Product Name	Size	Price	Quantity
L00657	eStain L1 Protein Staining Device	1 unit	Quote	1

Technical Resources

eStain® L1 Protein Staining System