eStain™ L1 Protein Staining System

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Catalog Products » Protein Electrophoresis » eStain™ L1 Protein Staining System

Overview

eStain is a highly efficient protein PAGE gel staining system, which uses Coomassie Brilliant Blue and a patented protein staining technology developed by Genscript. eStain staining system integrates the traditional three steps of fixing-staining-destaining into one and can stain/destain two protein PAGE gels simultaneously in 10 minutes or less.

eStain works with all types of precast as well as homemade PAGE gels. Compared with the conventional staining methods, gels stained by eStain have crisp blue bands with minimum or no background. eStain also offers superior sensitivity compared to conventional methods and can detect as low as 12.5 ng of protein.

Protein Electrophoresis and Blotting

Highlights

Automated fast staining

Consistent results for Coomassie Blue Staining

Membrane staining

Easy to use with clean and convenient operation

eStain Videos

Video

Introductory Video

Detailed Set Up and Operation Video

Why choose eStain™ L1

Shortened workflow increases your productivity

The eStain Protein Staining System combines three steps of traditional Coomassie blue staining method into one single step to greatly reduce the time required for protein staining (Table 1).

	Wash or Rinse Step	Stain Step	Destain or Rinse Step	Time saved with eStain L1
eStain™	0 min	Electric Staining <10 min	0 min
Staining reagent A	Rinse 15 min	Stain 1-3 hr	Wash 1-3 hr	2 hr , 15 min-6 hr, 15 min
Staining reagent B	Wash 15 min	Stain 1 hr	Rinse 30 min	1 hr , 45 min
Gelcode Blue Safe Protein Stain (Basic Protocol)	Wash 15 min	Stain 15 min-1 hr	Destain 1-2 hr	1 hr , 30 min - 3 hr , 15 min

Excellent sensitivity and repeatability with Coomassie Blue
- Gel: ExpressPlus™ PAGE Gel, 4-12%, 10 wells (GenScript M41210)
- Program: Stain
- Staining solution: Coomasie Blue
- Time: 9 min 30 s
Compatibility for membrane staining with Ponceau S solution
- Gel: SurePAGE™ Gel, Bis-Tris, 4-12% (GenScript, M00653)
- Membrane: GenScript nitrocellulose, PVDF membranes
- Samples: Pre-stained protein standard (GenScript, M006245); unstained PAGE-Master protein standard (GenScript, M00516)
- Program: Stain
- Staining Solution: Ponceau S solution
- Time: 9 min 30 s

Quick Procedures

eStain Protein Staining device quick guide

Note: For complete instructions and advanced programming, please refer to the Manual

Operational Instructions
Step 1. Add distilled water into the tray provided. When gel electrophoresis is complete, carefully remove the gel from the gel cassette and briefly rinse the gel with distilled water for 1 min
Step 2. Open the gel holder and place it on the table with the fabric side down. Carefully place the rinsed gel on the fabric side of the gel holder. Ensure that the upper part of the gel (with comb) point to the axis of the gel holder and is as close to the axis as possible
Step 3. Pre-wet a piece of filter paper (provided) with distilled water and place it on top of the gel
Step 4. Close the gel holder
Step 5. Choose a Channel and insert the gel holder into the channel. *Note：Please ensure that the fabric side of the gel holder is facing the user before inserting it into the Channel*
Step 6. Press the corresponding channel to start the program. The channel timer starts to countdown
Step 7. The instrument beeps as the program countdown to 0
Step 8. The channel stops flashing and the screen returns to normal. Take out the gel holder and the stained gel. The staining process is complete.

Product List

Device Ordering
Consumables Ordering
Extended Warranty

eStain™ Protein Staining Device

Cat. No.	Product Name	Quantity	Price	Order
L00657	eStain L1 Protein Staining Device		- 1 unit

eStain Kits

Cat. No.	Product Name	Quantity	Price	Order
L00753	eStain L1C Protein Staining Kit		$106.00 1 Unit

Accessories

Cat. No.	Product Name	Quantity	Price	Order
L00658	eStain Gel Holder		$361.00 2 unit
L00661	Liquid container		$51.00 1 unit (10L)

eStain Warranty Statement

Cat. No.	Description	Price
W00004-2	Extended 1-yr warranty for eStain, 2nd year or 20000 min	Quote
W00004-3	Extended 1-yr warranty for eStain, 3rd year or 30000 min	Quote
W00004-4	Extended 1-yr warranty for eStain, 4th year or 40000 min	Quote