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Catalog Products » Molecular Biology Reagents » PCR Reagents » Taq DNA Polymerase without Mg2+

Taq DNA Polymerase without Mg2+

Description DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA polymerase is the most common polymerase used for PCR.
Key Features
  • Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
  • High purity: No contamination activity has been detected in standard test reactions.

QC
QC Tests
PCR* performance, activity, nuclease

Lane Taq Unit used
1Leading
Brand A
0.1
2 0.25
3 0.5
4Leading
Brand B
0.1
5 0.25
6 0.5
7GenScript0.1
8 0.25
9 0.5


Note
Formulation
GenScript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.

Unit Definition
One unit is the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

10 X Reaction Buffer (without Mg2+)
500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.
Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
Storage Buffer and Concentration
Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

Storage:
-20°C

*The PCR process is covered by US. Patents numbers 4683195 and 4683202, issued to Cetus and owned by Hoffman-La Roche Inc. GenScript does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for persons who either have a license to perform PCR or are not required to obtain a license. Sale of this product is restricted to regions or countries where native Taq DNA polymerase patents have been invalidated.

  • PCR*
  • 3' A-tailing of blunt ends
  • Primer extension
  • DNA sequencing




Cat. No.
Product Name
Price
E00007-1000
Taq DNA Polymerase - 1000 U (1000.0U/vial)
$60.00
E00007-50000
Taq DNA Polymerase - 50000 U (1000.0U/vial)
$2700.00
 


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Cat. No. E00008
Size
Price
1000 U (1000.0U/vial) $60.00
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