Congratulations to Jeffrey C. Hall, Michael Rosbash and Michael W. Young, who are rewarded the 2017 Nobel Prize in Physiology or Medicine for their discoveries of molecular mechanisms controlling the circadian rhythm. All the living organisms have a biological clock that helps them adapt to the regular rhythm of the day and further keep the life homeostasis. In summary, they jointly discovered three key clock genes, Period (PER), Timeless (TIM) and Doubletime (DBT), which play vital functions in controlling circadian rhythm.
To celebrate their awards and acknowledge their contributions on human health, GenScript launches a circadian rhythm related gene ORF clones promotion for all scientists. We are aiming to do our best to support scientific endeavors through offering high-quality products and services all the time.
Circadian rhythm gene ORF cDNA clones promotion list
Please send your desired ORF clones ID with related promotion code to our TAM for the discount price.
|Species||Gene symbol||Accession no.||Clone ID *||Vector **||Turnaround time ***||Price(USD)||Price after discount||Promotion code ****|
* One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.
** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).
*** Business Day
**** Please take note that 2017NPFree can be used once for each account number, 50% off for all the other clones.
In 1984, Jeffrey Hall and Michael Rosbash at Brandeis University identified and isolated the Period gene that encodes a protein named PER. They showed that protein PER level peaks at night and decline in daytime following 24-hour cycle. Michael Young at Rockefeller University independently discovered another monumental gene Timeless that encodes TIM protein involved in regulating PER’s cyclic production in cells. The TIM binds to PER and shuttle it from cytoplasm into its nucleus, where it can inhibit the expression of the period gene. Further studies revealed the DBT protein, a kinase that could phosphorylates PER to promote its degradation, triggering the transcription of the clock and clock-controlled genes. Consequently, the oscillations of PER and TIM proteins presence causes oscillations in themselves and other clock genes' expression, which is the basis for circadian rhythmicity.
Their work is very meaningful because the internal biological clock is now well recognized as a key feature of eukaryotic organisms from fruit flies to humans. The beauty biological clocks is that it allows organisms to adapt their physiology to the Earth’s associated rotation daily light cycles, including blood pressure, sleep, body temperature, and metabolism. Numerous evidence suggest that disruption of circadian rhythm may cause serious health problems, for instance, increased risk of cancer. Scientists are now looking to improve treatment of cardiovascular and other diseases by coordinating delivery of drugs with a patient’s clock.
Zehring, W.A., Wheeler, D.A., Reddy, P., Konopka, R.J., Kyriacou, C.P., Rosbash, M., and Hall, J.C. (1984). P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic Drosophila melanogaster. Cell 39, 369–376.
Bargiello, T.A., Jackson, F.R., and Young, M.W. (1984). Restoration of circadian behavioural rhythms by gene transfer in Drosophila. Nature 312, 752–754.
Siwicki, K.K., Eastman, C., Petersen, G., Rosbash, M., and Hall, J.C. (1988). Antibodies to the period gene product of Drosophila reveal diverse tissue distribution and rhythmic changes in the visual system. Neuron 1, 141–150.
Hardin, P.E., Hall, J.C., and Rosbash, M. (1990). Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levels. Nature 343, 536–540.
Liu, X., Zwiebel, L.J., Hinton, D., Benzer, S., Hall, J.C., and Rosbash, M. (1992). The period gene encodes a predominantly nuclear protein in adult Drosophila. J Neurosci 12, 2735–2744.
Vosshall, L.B., Price, J.L., Sehgal, A., Saez, L., and Young, M.W. (1994). Block in nuclear localization of period protein by a second clock mutation, timeless. Science 263, 1606–1609.
Price, J.L., Blau, J., Rothenfluh, A., Abodeely, M., Kloss, B., and Young, M.W. (1998). double-time is a novel Drosophila clock gene that regulates PERIOD protein accumulation. Cell 94, 83–95.
Michael Rosbash and Joseph S. Takahashi (2002). Circadian rhythms: The cancer connection. Nature 420, 373-374.
Terms and conditions:
- Only domestic (US) and international customers, excluding Japan and China, are eligible to attend this promotion.
- This promotion is valid until Dec 31, 2017, US-EST time zone.
- Please take note that 2017NPFree can be used on next-day clones once for each account number, 50% off for all the other clones.
- All next-day shipping ORF clones are expression-ready and delivered in the pcDNA3.1+/C-(K)-DYK vector.
- Customer is responsible for paying the shipping fee to receive the free next-day shipping and discounted ORF clone.
- All the clones can be cloned into any vectors, additional sub-cloning charge will be applied, please contact our TAM for quotation.
- Contact us at firstname.lastname@example.org if you have any questions about this promotion.