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CloneEZ® PCR Cloning Kit

*This product has been discontinued!*

Note: This product has been discontinued, and replaced by the GenBuilder Cloning kit. To avail a free sample of the GenBuilder Cloning kit, please contact [email protected].

GenScript CloneEZ® PCR Cloning Kit is designed for the quick PCR cloning , it is especially powerful in high-throughput cloning of PCR products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases.
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Description


Description
The GenScript CloneEZ® PCR Cloning Kit is designed for the quick PCR cloning , it is especially powerful in high-throughput cloning of PCR products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases. Using our proprietary CloneEZ® Enzyme, this kit is able to rapidly generate precise and directional constructs by carrying out 30-minute incubation at room temperature (20 °C-25 °C).
Product Introduction
Key Features
Key Features
The CloneEZ® PCR Cloning Kit offers flexible and quick directional cloning and enables users to:
  • Clone into any vector without any need for restriction enzymes, phosphatase treatments, or ligases
  • Provide up to 10 kb PCR cloning at any restriction site
  • Rapidly and precisely generate correctly oriented constructs and inserts
  • Work with PCR that employ any thermostable polymerases


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    Storage Store the kit at -20°C. It will remain stable for at least one year, if stored properly.
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    Applications
    Applications
    The GenScript CloneEZ® PCR Cloning Kit can be used in a variety of applications:
  • PCR cloning
  • HTP PCR cloning
  • Joining of DNA fragments

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    Citations
    Liu HY, et al. Acetylation of MORC2 by NAT10 regulates cell-cycle checkpoint control and resistance to DNA-damaging chemotherapy and radiotherapy in breast cancer. Nucleic Acids Res. ( 2020-02)
    Jimmy Massenet, et al. Derivation and Characterization of Immortalized Human Muscle Satellite Cell Clones from Muscular Dystrophy Patients and Healthy Individuals. Cells. (2020)
    PremnathPriyanka, et al. Screening for inhibitors of mutacin synthesis in Streptococcus mutans using fluorescent reporter strains. BMC Microbiol. (2018)
    JoshiAmit S, et al. Lipid droplet and peroxisome biogenesis occur at the same ER subdomains. Nat Commun. (2018)
    De Santi Chiara, et al. Identification of MiR-21-5p as a Functional Regulator of Mesothelin Expression Using MicroRNA Capture Affinity Coupled with Next Generation Sequencing. PLoS ONE. (2017)
    Subramanian A, et al. Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions. Elife. (2014)
    Tang L, et al. Different endocytic functions of AGEF-1 in C. elegans coelomocytes. Biochim Biophys Acta. (2012-07)