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Catalog Products » Molecular Biology Reagents » PCR Cloning » CloneEZ® PCR Cloning Kit

CloneEZ® PCR Cloning Kit

*This product has been discontinued!*
Description
Description
The GenScript CloneEZ® PCR Cloning Kit is designed for the quick PCR cloning , it is especially powerful in high-throughput cloning of PCR products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases. Using our proprietary CloneEZ® Enzyme, this kit is able to rapidly generate precise and directional constructs by carrying out 30-minute incubation at room temperature (20 °C-25 °C).

Key Features
Key Features
The CloneEZ® PCR Cloning Kit offers flexible and quick directional cloning and enables users to:
  • Clone into any vector without any need for restriction enzymes, phosphatase treatments, or ligases
  • Provide up to 10 kb PCR cloning at any restriction site
  • Rapidly and precisely generate correctly oriented constructs and inserts
  • Work with PCR that employ any thermostable polymerases



  • Storage Store the kit at -20°C. It will remain stable for at least one year, if stored properly.

    Applications
    The GenScript CloneEZ® PCR Cloning Kit can be used in a variety of applications:
  • PCR cloning
  • HTP PCR cloning
  • Joining of DNA fragments




    Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

    Hui JZ, et al. Facile Method for the Site‐Specific, Covalent Attachment of Full‐Length IgG onto Nanoparticles. Small. 2014April;

    A Elias, et al. Quantitative Comparison of Tumor Delivery for Multiple Targeted Nanoparticles Simultaneously by Multiplex ICP-MS. Sci Rep. 2014July;45840

    Q Wang, et al. Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio). PLoS One. 2014July;9(7):e102492

    Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

    Hui JZ, et al. Facile Method for the Site‐Specific, Covalent Attachment of Full‐Length IgG onto Nanoparticles. Small. 2014April;

    Wang X., et al. Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4021-6.

    Chen Y., et al. Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach. Environ Sci Technol. 2013 Aug 20;47(16):9355-62.

    Dondelinger Y., et al. RIPK3 contributes to TNFR1-mediated RIPK1 kinase-dependent apoptosis in conditions of cIAP1/2 depletion or TAK1 kinase inhibition. Cell Death Differ. 2013 Oct;20(10):1381-92.

    Glenn TD., et al. Analysis of Gpr126 function defines distinct mechanisms controlling the initiation and maturation of myelin. Development. 2013 Aug;140(15):3167-75.

    Nie C., et al. Production and secretion of Lactobacillus crispatus β-galactosidase in Pichia pastoris. Protein Expr Purif. 2013 Nov;92(1):88-93.

    Revilla-Guarinos A., et al. Characterization of a Regulatory Network of Peptide Antibiotic Detoxification Modules in Lactobacillus casei BL23. Appl Environ Microbiol. 2013 May;79(10):3160-70.

    You can visit "Citations Database" for more citations.




    For more documents, please visit "Technical Support".

    Note: This product has been discontinued, and replaced by the GenBuilder Cloning kit. To avail a free sample of the GenBuilder Cloning kit, please contact [email protected].

    Cat. No. L00339