Catalog Products » Stable Cell Lines » Human Recombinant Endothelin Receptor Type B Stable Cell Line
CHO-K1/EDNRB Stable Cell Line

Figure 1: ET-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB cells. The cells were loaded with Calcium-4 prior to stimulation with an EDNRB receptor agonist ET-1. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ET-1 (Mean ± SD, n = 2). The EC50 of ET-1 on EDNRB expressing CHO-K1 cells was 41.5 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/EDNRB Stable Cell Line

In mammals, the endothelin (ET) family comprises three endogenous isoforms: ET-1, ET-2, and ET-3. These endothelium-derived peptides perform their functions via two endothelin receptors, classified as EDNRA and EDNRB. EDNRB receptors present on the endothelial cells lining the vessel wall and play a role in the release of endothelium-derived relaxing factors such as nitric oxide (NO) and prostanoids and in removing ET from the circulation.
M00150
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Description

In mammals, the endothelin (ET) family comprises three endogenous isoforms: ET-1, ET-2, and ET-3. These endothelium-derived peptides perform their functions via two endothelin receptors, classified as EDNRA and EDNRB. EDNRB receptors present on the endothelial cells lining the vessel wall and play a role in the release of endothelium-derived relaxing factors such as nitric oxide (NO) and prostanoids and in removing ET from the circulation.

Synonyms

ETB receptor, HSCR2, HSCR, ETB, Ednra, ET-B, ET-BR, endothelin B receptor, endothelin receptor, endothelin receptor non-selective type, endothelin receptor type B, endothelin-B receptor, Sox10m1, ETR-b, AU022549

Overview
Applications Functional assay for EDNRB receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)

Examples
  • CHO-K1/EDNRB Stable Cell Line
  • CHO-K1/EDNRB Stable Cell Line

    Figure 1: ET-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB cells. The cells were loaded with Calcium-4 prior to stimulation with an EDNRB receptor agonist ET-1. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ET-1 (Mean ± SD, n = 2). The EC50 of ET-1 on EDNRB expressing CHO-K1 cells was 41.5 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to Background Ratio (S/B) = Top/Bottom


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