Catalog Products » Stable Cell Lines » Human Recombinant Muscarinic Acetylcholine Receptor M1 Stable Cell Line
CHO-K1/M1 Stable Cell Line

Figure 2. 10 μg of membranes prepared from CHO-K1 cells stably expressing M1 receptors were incubated with indicated concentrations of [3H]N-Methylscopolamine ([3H]NMS) in the absence (total binding) or presence of 1000-fold access unlabeled Atropine (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

CHO-K1/M1 Stable Cell Line

Figure 3. 10 μg of membranes prepared from CHO-K1 cells stably expressing M1 receptors were incubated with indicated concentrations of Atropine in the presence of 0.2 nM [3H]N-Methylscopolamine ([3H]NMS). Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

CHO-K1/M1 Stable Cell Line

Figure 1: Carbachol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the M1 receptor agonist, carbachol. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of carbachol (Mean ± SD, n = 2). The EC50 of carbachol on this cell was 31.6 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/M1 Stable Cell Line

CHO-K1/M1 Stable Cell Line

M1 was expressed in the CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system and esophageal smooth muscle. Synaptic transmission by muscarinic acetylcholine receptors (mAChRs) is employed throughout the central and peripheral nervous systems to elicit a large and diverse array of neurophysiological actions. An important aspect of mAChR functional diversity is reflected by the multitude of biochemical and electrophysiological actions evoked by acetylcholine binding to mAChRs, which include the regulation of intracellular levels of cAMP, cGMP and inositol phospholipids, and the opening or closing of the potassium, calcium, and chloride ion channels found in certain tissues.
M00185
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Description

M1 was expressed in the CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system and esophageal smooth muscle. Synaptic transmission by muscarinic acetylcholine receptors (mAChRs) is employed throughout the central and peripheral nervous systems to elicit a large and diverse array of neurophysiological actions. An important aspect of mAChR functional diversity is reflected by the multitude of biochemical and electrophysiological actions evoked by acetylcholine binding to mAChRs, which include the regulation of intracellular levels of cAMP, cGMP and inositol phospholipids, and the opening or closing of the potassium, calcium, and chloride ion channels found in certain tissues.

Synonyms

M1 receptor, m1, acetylcholine receptor, muscarinic 1, Cholinergic receptor muscarin 1, M1 muscarinic acetylcholine receptor, cholinergic receptor, muscarinic 1, m1 muscarinic acetylcholine receptor protein, muscarinic acetylcholine receptor M1, muscarinic acetylcholine receptor 1, Chrm-1, M1R, AW495047

Overview
Applications Functional assay for M1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 ug/ml Zeocin (Cat. #R250-01, Life Technologies)

Examples
  • CHO-K1/M1 Stable Cell Line
  • CHO-K1/M1 Stable Cell Line

    Figure 2. 10 μg of membranes prepared from CHO-K1 cells stably expressing M1 receptors were incubated with indicated concentrations of [3H]N-Methylscopolamine ([3H]NMS) in the absence (total binding) or presence of 1000-fold access unlabeled Atropine (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

  • CHO-K1/M1 Stable Cell Line
  • CHO-K1/M1 Stable Cell Line

    Figure 3. 10 μg of membranes prepared from CHO-K1 cells stably expressing M1 receptors were incubated with indicated concentrations of Atropine in the presence of 0.2 nM [3H]N-Methylscopolamine ([3H]NMS). Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

  • CHO-K1/M1 Stable Cell Line
  • CHO-K1/M1 Stable Cell Line

    Figure 1: Carbachol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the M1 receptor agonist, carbachol. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of carbachol (Mean ± SD, n = 2). The EC50 of carbachol on this cell was 31.6 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to Background Ratio (S/B) = Top/Bottom

  • CHO-K1/M1 Stable Cell Line
  • CHO-K1/M1 Stable Cell Line


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