Catalog Products » Stable Cell Lines » Human Recombinant KiSS1-derived Peptide Receptor Stable Cell Line
CHO-K1/KiSS1 Stable Cell Line

Figure 1: Kisspeptin-10-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/KiSS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with KiSS1 receptor agonist, Kisspeptin-10. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of LPA (Mean ± SD, n = 2). The EC50 of LPA on CHO-K1/KiSS1 cells was 14.2 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/KiSS1 Stable Cell Line

The KiSS1-derived peptide receptor (also known as GPR54 or the Kisspeptin receptor) is a Gq-coupled receptor which binds the peptide hormone kisspeptin (metastin). The KiSS1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS1 in inhibition of metastasis in human cancer. Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus. In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism.
M00190
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Description

The KiSS1-derived peptide receptor (also known as GPR54 or the Kisspeptin receptor) is a Gq-coupled receptor which binds the peptide hormone kisspeptin (metastin). The KiSS1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS1 in inhibition of metastasis in human cancer. Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus. In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism.

Synonyms

KISS1R, GPR54, Metastin receptor, AXOR12, OT7T175

Overview
Applications Functional assay for KiSS1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/KiSS1 Stable Cell Line
  • CHO-K1/KiSS1 Stable Cell Line

    Figure 1: Kisspeptin-10-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/KiSS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with KiSS1 receptor agonist, Kisspeptin-10. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of LPA (Mean ± SD, n = 2). The EC50 of LPA on CHO-K1/KiSS1 cells was 14.2 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.


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