Figure 1: Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, neurotensin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 2). The EC50 of neurotensin on this cell was 7.4 nM.
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom
CHO-K1/NTS1 Stable Cell Line
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