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Figure 1. substance-P (SAR 9)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK1 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK1 receptor agonist, substance-P (SAR 9). The intracellular calcium change was measured by FLIPR. The effects of agonist (%Stimulation) were plotted against the log of the cumulative doses (5-fold dilution) of substance-P (SAR 9) (Mean ± SD, n = 4). The EC50 of substance-P (SAR 9) on CHO-K1/NK1 in CHO-K1 cells was 6.15 nM. The S/B of substance-P (SAR 9) on NK1 in CHO-K1 cells was 7.5.
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