|Synonyms||Human Recombinant Muscarinic Acetylcholine Receptor M4 Stable Cell Line|
|Description||Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M4 muscarinic receptor couples to Gi/o to inhibit cAMP production. GenScript co-transfected human M4 with Gα15 in the CHO-K1 which supports high levels of recombinant M4 expression on the cell surface and contains high levels of Gα15 to couple the receptor to the calcium signaling pathway.|
|Culture Medium||Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin|
|Freeze Medium||45% culture medium, 45% FBS, and 10% DMSO|
|Storage||Liquid nitrogen immediately upon delivery|
|Functional assays for M4 receptor
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