Catalog Products » Stable Cell Lines » Human Recombinant MC3 Melanocortin Receptor Stable Cell Line
CHO-K1/MC3/Gα15 Stable Cell Line

Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC3/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an MC3 receptor agonist, ACTH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SD, n = 2). The EC50 of ACTH on this cell was 0.43 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/MC3/Gα15 Stable Cell Line

The melanocortin receptor 3, MC3 receptor, is Gs-coupled GPCRs expressed in brain, placental, and gut tissues but not in melanoma cells or in the adrenal gland. MC3 receptor knockouts exhibit a metabolic syndrome. At 4-6 months old, MC3 receptor knockout mice show increased fat mass, reduced lean mass and a higher feed efficiency, with normal metabolic rates. MC3 receptor knockout mice are hyperleptinaemic and males are often mildly hyperinsulinaemic.
M00242
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Description

The melanocortin receptor 3, MC3 receptor, is Gs-coupled GPCRs expressed in brain, placental, and gut tissues but not in melanoma cells or in the adrenal gland. MC3 receptor knockouts exhibit a metabolic syndrome. At 4-6 months old, MC3 receptor knockout mice show increased fat mass, reduced lean mass and a higher feed efficiency, with normal metabolic rates. MC3 receptor knockout mice are hyperleptinaemic and males are often mildly hyperinsulinaemic.

Synonyms

MC3R, MC3, MC3-R, melanocortin 3 receptor, BMIQ9, OB20, OQTL

Overview
Applications Functional assay for MC3 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

Examples
  • CHO-K1/MC3/Gα15 Stable Cell Line
  • CHO-K1/MC3/Gα15 Stable Cell Line

    Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC3/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with an MC3 receptor agonist, ACTH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SD, n = 2). The EC50 of ACTH on this cell was 0.43 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom


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