Catalog Products » Stable Cell Lines » Human Recombinant 5-HT2C Serotonin Receptor Stable Cell Line
CHO-K1/5-HT2C Stable Cell Line

Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2C cells. The cells were loaded with Calcium-4 prior to being stimulated with 5-HT. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on cells was 1.5 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/5-HT2C Stable Cell Line

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT2C is expressed in the brain and spinal cord, especially the choroid plexus. 5-HT2C receptor agonists may have important clinical value in the treatment of mental and eating disorders, such as depression, panic anxiety, OCD, bulimia, and obesity. GenScript's cloned human 5-HT2C-expressing cell line is generated in the CHO-K1 host.
M00253
$8,500.00

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Description

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT2C is expressed in the brain and spinal cord, especially the choroid plexus. 5-HT2C receptor agonists may have important clinical value in the treatment of mental and eating disorders, such as depression, panic anxiety, OCD, bulimia, and obesity. GenScript's cloned human 5-HT2C-expressing cell line is generated in the CHO-K1 host.

Synonyms

5-HT2C receptor, 5-HT1C, HTR1C, 5-HT2C, 5-hydroxytryptamine (serotonin) receptor 2C, 5-HTR2C, 5HT-1C, 5-HT-1C, 5-HT1C, 5-hydroxytryptamine receptor 1C, 5-hydroxytryptamine receptor 2C, serotonin 1c receptor, serotonin receptor 2C, 5-HT2C receptor, 5-HT2cR, 5HT1c, SR1

Overview
Applications Functional assay for 5-HT2C receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/5-HT2C Stable Cell Line
  • CHO-K1/5-HT2C Stable Cell Line

    Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2C cells. The cells were loaded with Calcium-4 prior to being stimulated with 5-HT. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on cells was 1.5 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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