Catalog Products » Stable Cell Lines » Human Recombinant Gα15 Stable Cell Line

CHO-K1/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human G protein subunit alpha 15 (Gα15) in the cytoplasm. This protein enables G protein-coupled receptor binding activity and acts upstream of or within with a positive effect on calcium-mediated signaling.
M00257
$8,500.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human G protein subunit alpha 15 (Gα15) in the cytoplasm. This protein enables G protein-coupled receptor binding activity and acts upstream of or within with a positive effect on calcium-mediated signaling.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.