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1321N1/P2Y12/Gα15 Stable Cell Line

Figure 1: ADP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y12/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y12 receptor agonist, ADP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ADP (Mean ± SEM, n = 3). The EC50 of ADP on 1321N1/P2Y12/Gα15 cells was 78.85 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

1321N1/P2Y12/Gα15 Stable Cell Line

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Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y12 (P2Y12) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00261
$8,500.00

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Product Introduction
Product Description Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y12 (P2Y12) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 50 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium DMEM, 10% FBS
Freeze Medium-DATA 45% DMEM (Cat. No. 10569, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • 1321N1/P2Y12/Gα15 Stable Cell Line
    • 1321N1/P2Y12/Gα15 Stable Cell Line

    Figure 1: ADP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y12/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y12 receptor agonist, ADP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ADP (Mean ± SEM, n = 3). The EC50 of ADP on 1321N1/P2Y12/Gα15 cells was 78.85 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.