Figure 1. CCK-8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CCKA cells. The cells were loaded with Calcium-4 prior to stimulation with a CCKA receptor agonist, CCK-8. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of CCK-8 (Mean ± SD, n = 2). The EC50 of CCK-8 on this cell was 0.45 nM.
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/CCKA/Gα15 Stable Cell Line
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